Debat Humberto J, Grabiele Mauro, Aguilera Patricia M, Bubillo Rosana, Zapata Pedro D, Marti Dardo A, Ducasse Daniel A
Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), X5020ICA, Córdoba, Argentina,
Virus Genes. 2014 Oct;49(2):348-50. doi: 10.1007/s11262-014-1096-2. Epub 2014 Jun 26.
We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5'UTR and a 61 nt 3'UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop.
我们首次报告了一种感染亚热带木本作物马黛茶（巴拉圭冬青）的病毒。通过总RNA纯化，接着进行新一代测序、转录本组装和注释，鉴定出一种感染马黛茶的新型内质网病毒。线性双链RNA病毒基因组的完整序列长13,954个核苷酸，包含一个13,743个核苷酸的单一开放阅读框（ORF），有一个149个核苷酸的5'非翻译区（UTR）和一个61个核苷酸的3'UTR。预测的ORF编码一个4,581个氨基酸的多肽，具有一个UDP-葡萄糖糖基转移酶、一个荚膜多糖合成蛋白和一个RNA依赖的RNA聚合酶结构域。建议将所鉴定的病毒命名为马黛茶内质网病毒。鉴于该病毒家族的有趣特性，以及完全缺乏关于马黛茶病毒的文献，我们认为此处报告的信息将有助于引起对这一重要主题和作物的新的且必要的关注。
