Bejerman Nicolás, de Breuil Soledad, Debat Humberto, Miretti Marcos, Badaracco Alejandra, Nome Claudia
Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Camino 60 Cuadras Km 5,5, X5020ICA, Córdoba, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (1425 FBQ) CABA, Buenos Aires, Argentina.
Arch Virol. 2017 Aug;162(8):2481-2484. doi: 10.1007/s00705-017-3363-8. Epub 2017 Apr 27.
We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3'-N-P-P3-P4-M-G-L-5'. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named "yerba mate chlorosis-associated virus" (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.
我们展示了一种感染阿根廷马黛茶（巴拉圭冬青）的新病毒的分子特征。对呈现褪绿线性图案、褪绿环和叶脉黄化症状的患病马黛茶植株进行深度测序，结果鉴定出一种在序列和基因组结构上类似于植物弹状病毒的新病毒。我们已确定该病毒的完整基因组序列，其长度为12,876个核苷酸。在负义单链病毒RNA的反基因组方向上鉴定出七个开放阅读框（ORF），顺序为3'-N-P-P3-P4-M-G-L-5'。系统发育分析表明，所描述的病毒是细胞质弹状病毒属的一个新成员，这一结论得到了在受感染马黛茶细胞细胞质内观察到的类似弹状病毒颗粒的支持。该病毒被暂定命名为“马黛茶褪绿相关病毒”（YmCaV）。YmCaV基因组序列的可得性将有助于评估该病毒的遗传变异性，并确定其在这种马黛茶病害中的作用。
