Barkov A M, Perov V Iu, Pushkar' V G
Zh Mikrobiol Epidemiol Immunobiol. 1989 Jan(1):6-9.
Antisera to the antigens of 5 fractions, isolated as the result of the separation of P. pseudomallei aqueous saline extract by continuous electrophoresis in the vertical block of granulated gel, have been obtained. Immunoelectrophoresis with the use of P. pseudomallei aqueous saline extract has revealed that antisera to electrophoretic fractions contain antibodies mainly to the antigens of the corresponding fractions, which shows that this technique ensures the effective separation of P. pseudomallei biopolymers by their electrophoretic motility and molecular weight. These antisera differ in their species specificity. Thus, antisera to antigens with anode motility have been found to contain antibodies mainly to P. pseudomallei antigens and antisera to electroneutral antigens or to those with cathode motility, to P. pseudomallei and P. mallei antigens.
已获得针对5种组分抗原的抗血清，这些组分是通过在粒状凝胶垂直块中进行连续电泳分离类鼻疽杆菌水盐提取物而得到的。使用类鼻疽杆菌水盐提取物进行免疫电泳表明，针对电泳组分的抗血清主要含有针对相应组分抗原的抗体，这表明该技术可通过其电泳迁移率和分子量有效分离类鼻疽杆菌生物聚合物。这些抗血清在种属特异性方面存在差异。因此，已发现针对具有阳极迁移率抗原的抗血清主要含有针对类鼻疽杆菌抗原的抗体，而针对电中性抗原或具有阴极迁移率抗原的抗血清则含有针对类鼻疽杆菌和鼻疽杆菌抗原的抗体。
