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揭示负责古菌 rudivirus 进入的细胞表面蛋白和 IV 型分泌蛋白。

Unveiling cell surface and type IV secretion proteins responsible for archaeal rudivirus entry.

作者信息

Deng Ling, He Fei, Bhoobalan-Chitty Yuvaraj, Martinez-Alvarez Laura, Guo Yang, Peng Xu

机构信息

Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark.

Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark

出版信息

J Virol. 2014 Sep 1;88(17):10264-8. doi: 10.1128/JVI.01495-14. Epub 2014 Jun 25.

Abstract

Sulfolobus mutants resistant to archaeal lytic virus Sulfolobus islandicus rod-shaped virus 2 (SIRV2) were isolated, and mutations were identified in two gene clusters, cluster sso3138 to sso3141 and cluster sso2386 and sso2387, encoding cell surface and type IV secretion proteins, respectively. The involvement of the mutations in the resistance was confirmed by genetic complementation. Blocking of virus entry into the mutants was demonstrated by the lack of early gene transcription, strongly supporting the idea of a role of the proteins in SIRV2 entry.

摘要

分离出了对古菌裂解病毒冰岛硫化叶菌杆状病毒2(SIRV2)具有抗性的硫化叶菌突变体,并在两个基因簇中鉴定到了突变,这两个基因簇分别是编码细胞表面蛋白的sso3138至sso3141基因簇以及编码IV型分泌蛋白的sso2386和sso2387基因簇。通过基因互补证实了这些突变与抗性有关。早期基因转录缺失证明病毒进入突变体受到阻碍,这有力地支持了这些蛋白质在SIRV2进入过程中发挥作用的观点。

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