广谱中和抗体VRC01可防止HIV-1从浆细胞样树突状细胞传播至CD4 T淋巴细胞。
Broadly neutralizing antibody VRC01 prevents HIV-1 transmission from plasmacytoid dendritic cells to CD4 T lymphocytes.
作者信息
Su Bin, Lederle Alexandre, Laumond Géraldine, Ducloy Camille, Schmidt Sylvie, Decoville Thomas, Moog Christiane
机构信息
INSERM U1109, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France
INSERM U1110, Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Université de Strasbourg, Strasbourg, France.
出版信息
J Virol. 2014 Sep;88(18):10975-81. doi: 10.1128/JVI.01748-14. Epub 2014 Jun 25.
Abstract
Plasmacytoid dendritic cells (pDC) poorly replicate human immunodeficiency virus type 1 (HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and increases pDC maturation and alpha interferon (IFN-α) secretion. HIV-1 transfer to T lymphocytes is inhibited by broadly neutralizing antibody VRC01 with efficiency similar to that of cell-free infection of T lymphocytes. Interestingly, prevention of HIV-1 transmission by VRC01 retains IFN-α secretion. These results emphasize the multiple functions of VRC01 in protection against HIV-1 acquisition.
摘要
浆细胞样树突状细胞（pDC）对1型人类免疫缺陷病毒（HIV-1）的复制能力较弱，但能有效地将HIV-1传递给相邻的CD4 T淋巴细胞。我们发现，与T淋巴细胞共培养会下调SAMHD1的表达，增强HIV-1的复制，并增加pDC的成熟和α干扰素（IFN-α）的分泌。广泛中和抗体VRC01对HIV-1传递给T淋巴细胞具有抑制作用，其效率与T淋巴细胞的无细胞感染相似。有趣的是，VRC01对HIV-1传播的预防作用能保留IFN-α的分泌。这些结果强调了VRC01在预防HIV-1感染方面具有多种功能。
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