NS3的连接区在丙型肝炎病毒的复制和感染性中起着关键作用。
The linker region of NS3 plays a critical role in the replication and infectivity of hepatitis C virus.
作者信息
Kohlway Andrew, Pirakitikulr Nathan, Ding Steve C, Yang Feng, Luo Dahai, Lindenbach Brett D, Pyle Anna M
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA.
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, USA.
出版信息
J Virol. 2014 Sep;88(18):10970-4. doi: 10.1128/JVI.00745-14. Epub 2014 Jun 25.
Abstract
Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.
摘要
丙型肝炎病毒（HCV）NS3-4A是病毒复制和组装所必需的。我们确定病毒组装对NS3-4A解旋酶和蛋白酶结构域之间连接区的突变敏感。然而，我们发现NS3的蛋白酶切割、RNA结合和解旋速率在体外受到的影响最小。因此，我们得出结论，NS3连接区对于介导蛋白质-蛋白质相互作用和动态控制至关重要，而不是调节NS3-4A的酶功能。
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