Ragheb H S, Ridge S, Din A
Analyst. 1989 Jan;114(1):57-60. doi: 10.1039/an9891400057.
Standard additions experiments for chlortetracycline hydrochloride and virginiamycin at a ratio of 16:1 showed a positive bias for both the plate and turbidimetric methods. The bias was eliminated by anion-exchange chromatography in both the presence and absence of feed components. When chlortetracycline and virginiamycin pre-mixes were used for fortification of laboratory-prepared feeds at chlortetracycline to virginiamycin ratios of 8 or 16:1, the recovery of virginiamycin, without treatment, was 93-100% using the plate assay and 181-236% with the turbidimetric method. The corresponding values obtained using anion-exchange chromatography were 89-99 and 85-91%, respectively. The anion-exchange technique is necessary if the turbidimetric method is used for the assay.
以16:1的比例对盐酸金霉素和维吉尼亚霉素进行标准加入实验，结果表明平板法和比浊法均存在正偏差。在有和没有饲料成分存在的情况下，通过阴离子交换色谱法均可消除该偏差。当使用金霉素和维吉尼亚霉素预混物以8或16:1的金霉素与维吉尼亚霉素比例强化实验室制备的饲料时，未经处理的情况下，平板法测定维吉尼亚霉素的回收率为93 - 100%，比浊法为181 - 236%。使用阴离子交换色谱法得到的相应值分别为89 - 99%和85 - 91%。如果使用比浊法进行测定，则阴离子交换技术是必要的。
