Mueller D L, Reed S J, Barkate J A
J Assoc Off Anal Chem. 1979 Jan;62(1):160-7.
A rapid turbidimetric assay has been developed for chlortetracycline hydrochloride (CTC-HCl) in finished animal feeds and feed supplements, using Leuconostoc mesenteroides as the test organism. Two modifications are presented: The incubation period of modification 1 is 2.5 hr and the sensitivity of the assay is 0.03 microgram CTC-HCl/assay tube. Modification 2 has a sensitivty of 0.01 microgram CTC-HCl/assay tube and requires an incubation period of 3.5 hr. For 21 feed formulations, the turbidimetric method recovered 95.7% of label claim. Recoveries of CTC-HCl standards from the same feeds ranged from 93.4 to 134% with a mean of 103%. The relative standard deviation among day-to-day duplicates is 3.50% for the faster modification and 1.63% for the more sensitive modification.
已开发出一种快速比浊法，用于测定成品动物饲料和饲料补充剂中的盐酸金霉素（CTC-HCl），使用嗜热链球菌作为测试微生物。提出了两种改进方法：方法1的孵育期为2.5小时，该测定法的灵敏度为0.03微克CTC-HCl/测定管。方法2的灵敏度为0.01微克CTC-HCl/测定管，需要3.5小时的孵育期。对于21种饲料配方，比浊法回收了标签声明量的95.7%。从相同饲料中回收的CTC-HCl标准品的回收率在93.4%至134%之间，平均为103%。对于较快的改进方法，日常重复测定之间的相对标准偏差为3.50%，对于更灵敏的改进方法为1.63%。
