Ragheb H S
J Assoc Off Anal Chem. 1976 May;59(3):536-9.
In previous experiments, the turbidimetric method for determining chlortetracycline-HCI (CTC-HCI) in feeds showed lower recovery than the AOAC plate method. Although the addition of vitamins to the turbidimetric medium improved results, values by the turbidimetric method remained about 10% lower than by the plate method. A modified (1.7X the weight recommended by the manufacturer) turbidimetric assay medium decreased assay sensitivity but did not significantly change the slope of S. aureus response to CTC-HCI. There was no evidence that vitamin fortification of the modified medium had any significant effect on the growth rate of test organism. Examination of about 100 samples of commercial feeds containing CTC-HCI showed excellent agreement in results between the turbidimetric and plate methods.
在之前的实验中，饲料中盐酸金霉素（CTC-HCI）的比浊法测定回收率低于美国官方分析化学师协会（AOAC）平板法。尽管向比浊法培养基中添加维生素可改善结果，但比浊法测得的值仍比平板法低约10%。一种改良的（制造商推荐重量的1.7倍）比浊法测定培养基降低了测定灵敏度，但并未显著改变金黄色葡萄球菌对CTC-HCI反应的斜率。没有证据表明改良培养基的维生素强化对测试微生物的生长速率有任何显著影响。对约100份含有CTC-HCI的商业饲料样本进行检测，结果表明比浊法和平板法之间的一致性良好。
