Talayero Beatriz, Wang Liyun, Furtado Jeremy, Carey Vincent J, Bray George A, Sacks Frank M
Department of Nutrition, Harvard School of Public Health, Boston, MA.
Channing Division of Network Medicine, Boston, MA.
J Lipid Res. 2014 Oct;55(10):2167-77. doi: 10.1194/jlr.M042333. Epub 2014 Jun 25.
Human HDLs have highly heterogeneous composition. Plasma concentrations of HDL with apoC-III and of apoE in HDL predict higher incidence of coronary heart disease (CHD). The concentrations of HDL-apoA-I containing apoE, apoC-III, or both and their distribution across HDL sizes are unknown. We studied 20 normal weight and 20 obese subjects matched by age, gender, and race. Plasma HDL was separated by sequential immunoaffinity chromatography (anti-apoA-I, anti-apoC-III, anti-apoE), followed by nondenaturing-gel electrophoresis. Mean HDL-cholesterol concentrations in normal weight and obese subjects were 65 and 50 mg/dl (P = 0.009), and total apoA-I concentrations were 119 and 118 mg/dl, respectively. HDL without apoE or apoC-III was the most prevalent HDL type representing 89% of apoA-I concentration in normal weight and 77% in obese (P = 0.01) individuals; HDL with apoE-only was 5% versus 8% (P = 0.1); HDL with apoC-III-only was 4% versus 10% (P = 0.009); and HDL with apoE and apoC-III was 1.5% versus 4.6% (P = 0.004). Concentrations of apoE and apoC-III in HDL were 1.5-2× higher in obese subjects (P ≤ 0.004). HDL with apoE or apoC-III occurred in all sizes among groups. Obese subjects had higher prevalence of HDL containing apoE or apoC-III, subfractions associated with CHD, whereas normal weight subjects had higher prevalence of HDL without apoE or apoC-III, subfractions with protective association against CHD.
人类高密度脂蛋白（HDL）的组成具有高度异质性。含有载脂蛋白C-III（apoC-III）的HDL的血浆浓度以及HDL中的载脂蛋白E（apoE）可预测冠心病（CHD）的更高发病率。含有apoE、apoC-III或两者的HDL-载脂蛋白A-I（apoA-I）的浓度及其在不同大小HDL中的分布情况尚不清楚。我们研究了20名体重正常和20名肥胖受试者，这些受试者在年龄、性别和种族方面相匹配。通过连续免疫亲和色谱法（抗apoA-I、抗apoC-III、抗apoE）分离血浆HDL，随后进行非变性凝胶电泳。体重正常和肥胖受试者的平均HDL胆固醇浓度分别为65和50mg/dl（P = 0.009），总apoA-I浓度分别为119和118mg/dl。不含apoE或apoC-III的HDL是最常见的HDL类型，在体重正常个体中占apoA-I浓度的89%，在肥胖个体中占77%（P = 0.01）；仅含apoE的HDL分别为5%和8%（P = 0.1）；仅含apoC-III的HDL分别为4%和10%（P = 0.009）；同时含有apoE和apoC-III的HDL分别为1.5%和4.6%（P = 0.004）。肥胖受试者HDL中apoE和apoC-III的浓度高1.5至2倍（P≤0.004）。含有apoE或apoC-III的HDL在各组的所有大小中均有出现。肥胖受试者中含有apoE或apoC-III的HDL（与CHD相关的亚组分）的患病率较高，而体重正常受试者中不含apoE或apoC-III的HDL（对CHD有保护作用的亚组分）的患病率较高。
