Guler Umut, Budak Yasemin, Ruh Emrah, Ocal Yesim, Canay Senay, Akyon Yakut
Department of Prosthodontics, Faculty of Dentistry, University of Hacettepe, Ankara, Turkiye.
Department of Microbiology, Faculty of Medicine, University of Hacettepe, Ankara, Turkiye.
Eur J Dent. 2013 Sep;7(Suppl 1):S054-S059. doi: 10.4103/1305-7456.119074.
The aim of this study was 2-fold. The first aim was to evaluate the effects of mixing technique (hand-mixing or auto-mixing) on bacterial attachment to polyether impression materials. The second aim was to determine whether bacterial attachment to these materials was affected by length of exposure to disinfection solutions.
Polyether impression material samples (n = 144) were prepared by hand-mixing or auto-mixing. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were used in testing. After incubation, the bacterial colonies were counted and then disinfectant solution was applied. The effect of disinfection solution was evaluated just after the polymerization of impression material and 30 min after polymerization. Differences in adherence of bacteria to the samples prepared by hand-mixing and to those prepared by auto-mixing were assessed by Kruskal-Wallis and Mann-Whitney U-tests. For evaluating the efficiency of the disinfectant, Kruskal-Wallis multiple comparisons test was used.
E. coli counts were higher in hand-mixed materials (P < 0.05); no other statistically significant differences were found between hand- and auto-mixed materials. According to the Kruskal-Wallis test, significant differences were found between the disinfection procedures (Z > 2.394).
The methods used for mixing polyether impression material did not affect bacterial attachment to impression surfaces. In contrast, the disinfection procedure greatly affects decontamination of the impression surface.
本研究有两个目的。第一个目的是评估混合技术（手工混合或自动混合）对细菌附着于聚醚印模材料的影响。第二个目的是确定细菌对这些材料的附着是否受消毒溶液暴露时间的影响。
通过手工混合或自动混合制备聚醚印模材料样本（n = 144）。使用大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌进行测试。培养后，对细菌菌落进行计数，然后施加消毒溶液。在印模材料聚合后立即以及聚合30分钟后评估消毒溶液的效果。通过Kruskal-Wallis检验和Mann-Whitney U检验评估手工混合制备的样本与自动混合制备的样本上细菌附着的差异。为评估消毒剂的效率，使用Kruskal-Wallis多重比较检验。
手工混合材料中的大肠杆菌计数较高（P < 0.05）；手工混合材料与自动混合材料之间未发现其他统计学上的显著差异。根据Kruskal-Wallis检验，消毒程序之间存在显著差异（Z > 2.394）。
用于混合聚醚印模材料的方法不影响细菌在印模表面的附着。相比之下，消毒程序对印模表面的去污有很大影响。
