Mohan Ajit George, Ebenezar A V Rajesh, Ghani Mohamed Fayas, Martina Leena, Narayanan Ashwin, Mony Bejoy
Director, Dental and Medical Centre, Cochin, Kerala, India.
Department of Conservative Dentistry and Endodontics, SRM Dental College, Chennai, Tamil Nadu, India.
Eur J Dent. 2014 Jan;8(1):118-123. doi: 10.4103/1305-7456.126264.
The aim of this study was to evaluate the surface/mineral changes on enamel before and after the application of acidulated phosphate fluoride (APF) gel, fluoride enhanced hydroxyapatite gel and propolis in conjunction with carbon-dioxide (CO2) laser.
Crowns of 40 human maxillary central incisors were collected and were divided into four groups of 10 each: Topical fluoride application only, topical fluoride application followed by CO2 laser irradiation, CO2 laser irradiation followed by topical fluoride application and CO2 laser irradiation before and after topical fluoride application. The 10 crowns in each group was again sectioned into four equal parts of mesio-incisal, disto-incisal, mesio-cervical and disto-cervical sections rendering 40 samples in each group. Each group was again subdivided into four subgroups: Subgroup C - untreated enamel surface (control). Subgroup A - APF gel application, subgroup R - fluoride enhanced hydroxyapatite gel application and subgroup P - propolis application. The surface morphology of the test samples were analyzed by scanning electron microscopy and mineral changes by energy dispersion X-ray spectrophotometer.
Total mineral content is maximum in Group 4A (CO2 laser irradiation before and after APF gel application) and calcium/phosphate ratio is highest in Group 4R (CO2 laser irradiation before and after Remin-Pro application). Group 2A (APF gel application followed by CO2 laser irradiation) has the maximum fluoride retention.
Laser irradiation of enamel through a topically applied APF gel is effective in the prophylaxis and management of dental caries.
本研究旨在评估应用酸化磷酸氟(APF)凝胶、氟增强羟基磷灰石凝胶和蜂胶结合二氧化碳(CO2)激光前后牙釉质的表面/矿物质变化。
收集40颗人上颌中切牙牙冠,分为四组,每组10颗:仅局部应用氟化物、局部应用氟化物后进行CO2激光照射、CO2激光照射后局部应用氟化物以及局部应用氟化物前后均进行CO2激光照射。每组的10颗牙冠再分别切成近中切端、远中切端、近中颈部和远中颈部四个相等部分,每组得到40个样本。每组再细分为四个亚组:C亚组 - 未处理的牙釉质表面(对照组)。A亚组 - 应用APF凝胶,R亚组 - 应用氟增强羟基磷灰石凝胶,P亚组 - 应用蜂胶。通过扫描电子显微镜分析测试样本的表面形态,通过能量色散X射线光谱仪分析矿物质变化。
4A组(APF凝胶应用前后均进行CO2激光照射)的总矿物质含量最高,4R组(Remin-Pro应用前后均进行CO2激光照射)的钙/磷比最高。2A组(应用APF凝胶后进行CO2激光照射)的氟保留量最大。
通过局部应用APF凝胶对牙釉质进行激光照射在预防和治疗龋齿方面是有效的。