Loiola Ana Bárbara de Araújo, Aires Carolina Patrícia, Curylofo-Zotti Fabiana Almeida, Rodrigues Junior Antônio Luiz, Souza-Gabriel Aline Evangelista, Corona Silmara Aparecida Milori
Department of Restorative Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto-SP, Brazil, 14040-904.
Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirão Preto-SP, Brazil, 14040-904.
J Lasers Med Sci. 2019 Summer;10(3):200-206. doi: 10.15171/jlms.2019.32. Epub 2019 Jul 6.
This study evaluated the impact of CO laser treatment and acidulated phosphate fluoride (APF) on enamel demineralization and biofilm formation, using and designs. Demineralized enamel slabs were distributed among 8 groups: placebo, placebo + continuous CO laser, placebo + repeated CO laser, placebo + ultrapulsed CO laser, 1.23% APF, APF + continuous CO laser, APF + repeated CO laser and APF + ultrapulsed CO laser. In the study, 15 enamel slabs from each group were subjected to a pH-cycling regimen for 14 days. In the cross over design, 11 volunteers wore palatal appliances with demineralized enamel slabs for 2 periods of 14 days each. Drops of sucrose solution were dripped onto enamel slabs 8×/day. Biofilms formed on slabs were collected and the colony-forming units (CFU) of and were determined. For both and studies, there was no significant difference between treatments (>0.05). However, all treatments increased microhardness of demineralized enamel (<0.05). After a further cariogenic challenge, with the exception of the placebo, all treatments maintained microhardness values (<0.05). Microbiological analysis showed no difference in (>0.05) or (>0.05) counts between groups. The results suggest that APF gel combined with the CO laser, regardless of the pulse emission mode used, was effective in controlling enamel demineralization, but none of the tested treatments was able to prevent bacterial colonization.
本研究采用[具体研究设计1]和[具体研究设计2]，评估了二氧化碳（CO）激光治疗和酸化磷酸氟（APF）对牙釉质脱矿和生物膜形成的影响。脱矿牙釉质片被分为8组：安慰剂组、安慰剂+连续CO激光组、安慰剂+重复CO激光组、安慰剂+超脉冲CO激光组、1.23% APF组、APF+连续CO激光组、APF+重复CO激光组和APF+超脉冲CO激光组。在[具体研究设计1]中，每组15片牙釉质片接受14天的pH循环方案。在交叉[具体研究设计2]中，11名志愿者佩戴带有脱矿牙釉质片的腭部矫治器，分两个阶段，每个阶段14天。每天8次将蔗糖溶液滴到牙釉质片上。收集牙釉质片上形成的生物膜，测定[细菌种类1]和[细菌种类2]的菌落形成单位（CFU）。对于[具体研究设计1]和[具体研究设计2]两项研究，各治疗组之间均无显著差异（P>0.05）。然而，所有治疗均提高了脱矿牙釉质的显微硬度（P<0.05）。在进一步的致龋刺激后，除安慰剂组外，所有治疗均维持了显微硬度值（P<0.05）。微生物分析表明，各组之间[细菌种类1]（P>0.05）或[细菌种类2]（P>0.05）的计数无差异。结果表明，无论使用何种脉冲发射模式，APF凝胶联合CO激光在控制牙釉质脱矿方面均有效，但所测试的治疗方法均无法预防细菌定植。
