Ranki A, Mattinen S, Yarchoan R, Broder S, Ghrayeb J, Lähdevirta J, Krohn K
Department of Dermatology, Helsinki University Central Hospital, Finland.
AIDS. 1989 Feb;3(2):63-9. doi: 10.1097/00002030-198902000-00002.
HIV-specific T-cell response in HIV-infected individuals at different stages of the disease and during zidovudine therapy was studied using HIV and HIV-envelope derived native and recombinant proteins as antigens. Neither antibody-negative at-risk individuals nor HIV-infected individuals responded to HIV or its envelope-derived proteins, even though they responded to a recall antigen, purified protein derivative of tuberculin (PPD). However, five out of 14 antibody- and antigen-negative sexual partners of known HIV-positive men did respond to HIV, native gp 120 and recombinant envelope and core proteins. Some AIDS-related complex (ARC) and AIDS patients treated with zidovudine also showed a low T-cell response which diminished along with clinical deterioration. A synthetic peptide representing one of the major T-cell epitopes in HIV envelope, frequently recognized by immunized and infected primates, gave only marginal stimulation in man. Our findings suggest that HIV infection in man results in a T-helper cell anergy directed against viral proteins. The response observed in the antibody- and antigen-negative sexual partners and in some of the zidovudine-treated patients implies that at least some epitopes on HIV envelope are immunogenic in man.
利用HIV及HIV包膜来源的天然和重组蛋白作为抗原，研究了处于疾病不同阶段以及接受齐多夫定治疗的HIV感染者的HIV特异性T细胞反应。抗体阴性的高危个体和HIV感染者均未对HIV或其包膜来源的蛋白产生反应，尽管他们对回忆抗原结核菌素纯蛋白衍生物（PPD）有反应。然而，在已知HIV阳性男性的14名抗体和抗原均阴性的性伴侣中，有5人确实对HIV、天然gp120以及重组包膜和核心蛋白产生了反应。一些接受齐多夫定治疗的艾滋病相关综合征（ARC）患者和艾滋病患者也表现出较低的T细胞反应，且随着临床病情恶化而减弱。一种代表HIV包膜中主要T细胞表位之一的合成肽，在免疫和感染的灵长类动物中经常被识别，但在人体中仅产生微弱的刺激。我们的研究结果表明，人类感染HIV会导致针对病毒蛋白的T辅助细胞无反应性。在抗体和抗原均阴性的性伴侣以及一些接受齐多夫定治疗的患者中观察到的反应意味着，HIV包膜上至少一些表位在人体中具有免疫原性。
