Parry J V, Mortimer P P
PHLS Virus Reference Laboratory, Central Public Health Laboratory, London, UK.
AIDS. 1989 Mar;3(3):173-6. doi: 10.1097/00002030-198903000-00008.
GACPAT is a modification of a gelatin particle-agglutination test for the detection of anti-HIV-1 and anti-HTLV-I. In it, antigen-coated particles react with immunoglobulin captured from the specimens onto the surface of U-bottomed reaction wells previously coated with anti-gamma chains. In initial tests on 1127 blood donations, of 628 HIV-1 high-risk specimens and 176 HTLV-I high-risk specimens, GACPAT for anti-HIV-1 was 98.9% specific and 100% sensitive, and for anti-HTLV-I was 99.0% specific and 93.0% sensitive. It rivalled the sensitivity of second-generation enzyme-linked immunosorbent assays (ELISA) for anti-HIV-1 in tests on specimen dilutions and on seroconversion series. GACPAT can be used as a cheap, large-scale test and as a simple confirmatory check on other assays.
GACPAT是一种用于检测抗HIV-1和抗HTLV-I的明胶颗粒凝集试验的改良方法。在该试验中，包被抗原的颗粒与从标本中捕获的免疫球蛋白发生反应，这些免疫球蛋白附着在先前包被有抗γ链的U型底反应孔表面。在对1127份血液捐献进行的初步检测中，在628份HIV-1高危标本和176份HTLV-I高危标本中，抗HIV-1的GACPAT特异性为98.9%，敏感性为100%；抗HTLV-I的GACPAT特异性为99.0%，敏感性为93.0%。在标本稀释试验和血清转化系列试验中，其对HIV-1的敏感性可与第二代酶联免疫吸附试验（ELISA）相媲美。GACPAT可用作一种廉价的大规模检测方法，也可作为对其他检测方法的一种简单确证检查。
