Parry J V, Connell J A, Reinbott P, Garcia A B, Avillez F, Mortimer P P
Hepatitis and Retrovirus Laboratory, Central Public Health Laboratory, London, United Kingdom.
J Med Virol. 1995 Jan;45(1):10-6. doi: 10.1002/jmv.1890450103.
A simple and cheap assay suitable for screening for anti-HIV 1 and anti-HIV 2 and discriminating between them was evaluated. In it specimens are incubated in U-bottomed microplate wells coated with anti-human IgG for 30 min at room temperature. After washing, 100 microliters of a 1 in 50 dilution of HIV 1-coated gelatin particles (Serodia-HIV 1/2, Fujirebio) are added. Settling patterns are read on the second day: A positive reaction is indicated by adherence of the particles and a negative by a button. The HIV 1 particles are then washed away and HIV 2 particles added. Anti-HIV 2 reaction patterns are read on the third day. To assess the performance of the modified "GACPAT HIV 1 + 2" assay a panel of 1,621 serum/plasma specimens was used. It comprised validated anti-HIV 1 positive (n = 220), anti-HIV 2 positive (n = 214), dual anti-HIV 1/anti-HIV 2 positive (n = 11), and anti-HIV negative (n = 1,176) serum/plasma specimens. All 434 specimens that contained anti-HIV 1 or anti-HIV 2 reacted positively with the homologous particles. The 11 dually positive specimens reacted positively with both HIV 1 and HIV 2 particles. Five (2.3%) anti-HIV 1 and five (2.3%) anti-HIV 2 positive specimens gave positive reactions with both particle types, but none of the five cross-reactive anti-HIV 2 specimens were dually reactive when the order of particle addition was reversed.(ABSTRACT TRUNCATED AT 250 WORDS)
对一种适用于筛查抗HIV-1和抗HIV-2并区分二者的简单廉价检测方法进行了评估。在该检测中，将标本在室温下于包被有抗人IgG的U型微孔板孔中孵育30分钟。洗涤后，加入100微升50倍稀释的包被HIV-1的明胶颗粒（Serodia-HIV 1/2，富士瑞必欧）。第二天读取沉淀模式：颗粒附着表明为阳性反应，形成纽扣状表明为阴性反应。然后洗去HIV-1颗粒，加入HIV-2颗粒。第三天读取抗HIV-2反应模式。为评估改良的“GACPAT HIV 1 + 2”检测方法的性能，使用了一组1621份血清/血浆标本。它包括经确认的抗HIV-1阳性（n = 220）、抗HIV-2阳性（n = 214）、抗HIV-1/抗HIV-2双阳性（n = 11）以及抗HIV阴性（n = 1176）的血清/血浆标本。所有434份含有抗HIV-1或抗HIV-2的标本均与同源颗粒发生阳性反应。11份双阳性标本与HIV-1和HIV-2颗粒均发生阳性反应。5份（2.3%）抗HIV-1阳性标本和5份（2.3%）抗HIV-2阳性标本与两种颗粒类型均发生阳性反应，但当颠倒颗粒添加顺序时，5份交叉反应性抗HIV-2标本中无一为双反应性。（摘要截短于250字）
