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从过江藤地上部分对黄酮类化合物进行生物测定指导的分离及抗氧化评估

Bioassay-guided isolation and antioxidant evaluation of flavonoid compound from aerial parts of Lippia nodiflora L.

作者信息

Sudha A, Srinivasan P

机构信息

Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu 630004, India.

出版信息

Biomed Res Int. 2014;2014:549836. doi: 10.1155/2014/549836. Epub 2014 May 25.

DOI:10.1155/2014/549836
PMID:24967379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4055635/
Abstract

The present study was designed to identify the DPPH (2, 2-diphenyl-1-picrylhydrazyl) free-radical scavenging constituents from methanol extract of L. nodiflora using bioassay-guided fractionation. The ethyl acetate fraction (EAF) revealed a strong antioxidant activity, compared to other fractions through in vitro DPPH radical-scavenging assay. The repeated fractionation of active EAF by silica gel column chromatography yielded a compound with strong antioxidant potential. The isolated bioactive compound was determined as 2-(3, 4-dimethoxyphenyl)-5-hydroxy-7-methoxy-4H-chromen-4-one (5-hydroxy-3', 4', 7-trimethoxyflavone), by comparing spectral data (UV, IR, (1)H NMR, (13)C NMR, and MS) with literature reports. The isolated compound demonstrated an excellent antioxidant activity through all antioxidant assays and also significantly inhibited lipid peroxidation at a concentration of 50 μg/mL. The results obtained suggested that extracts from L. nodiflora or its derived phytocompound can be used potentially as a bioactive source of natural antioxidants by contributing beneficial health effects.

摘要

本研究旨在通过生物活性导向分离法，从节节菜甲醇提取物中鉴定出清除DPPH（2,2-二苯基-1-苦基肼）自由基的成分。通过体外DPPH自由基清除试验，与其他组分相比，乙酸乙酯组分（EAF）显示出较强的抗氧化活性。对活性EAF进行硅胶柱色谱反复分离，得到一种具有较强抗氧化潜力的化合物。通过将光谱数据（紫外、红外、¹H NMR、¹³C NMR和质谱）与文献报道进行比较，确定分离得到的生物活性化合物为2-(3,4-二甲氧基苯基)-5-羟基-7-甲氧基-4H-色烯-4-酮（5-羟基-3',4',7-三甲氧基黄酮）。通过所有抗氧化试验，分离得到的化合物均表现出优异的抗氧化活性，并且在浓度为50 μg/mL时还能显著抑制脂质过氧化。所得结果表明，节节菜提取物或其衍生的植物化合物可能通过产生有益的健康影响，作为天然抗氧化剂的生物活性来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/df61af8d1f86/BMRI2014-549836.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/f19ac5bf24ef/BMRI2014-549836.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/df61af8d1f86/BMRI2014-549836.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/f19ac5bf24ef/BMRI2014-549836.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/8ba3e5f88602/BMRI2014-549836.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/9b688a49bb2c/BMRI2014-549836.003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/afa6b354ba75/BMRI2014-549836.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a90/4055635/df61af8d1f86/BMRI2014-549836.007.jpg

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