Silva Fabrício Souza, Menezes Pedro Modesto Nascimento, de Sá Pedro Guilherme Souza, Oliveira André Luís de Santana, Souza Eric Alencar Araújo, Bamberg Vinicius Martins, de Oliveira Henrique Ribeiro, de Oliveira Sheilla Andrade, Araújo Roni Evêncio E, Uetanabaro Ana Paula Trovatti, Silva Tânia Regina Dos Santos, Almeida Jackson Roberto Guedes da Silva, Lucchese Angélica Maria
Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, Avenida José de Sá Maniçoba, s/n, Centro, 56304-917 Petrolina, PE, Brazil ; Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina, s/n, Novo Horizonte, 44036-900 Feira de Santana, BA, Brazil.
Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, Avenida José de Sá Maniçoba, s/n, Centro, 56304-917 Petrolina, PE, Brazil.
Evid Based Complement Alternat Med. 2015;2015:463248. doi: 10.1155/2015/463248. Epub 2015 Mar 29.
The aim of this study was to evaluate crude extracts and fractions from leaves and stems of Lippia thymoides and to validate their use in folk medicine. In vitro antioxidant and antimicrobial activities and in vivo wound healing in rats, baker yeast-induced fever in young rats, and acute oral toxicity in mice assays were realized. The crude extracts and their dichloromethane and ethyl acetate fractions had potent radical-scavenging activity against the DPPH but were not effective in the β-carotene bleaching method. The dichloromethane fraction from the leaves extract showed the broadest spectrum of activity against S. aureus, B. cereus, and C. parapsilosis. The animals treated with crude extracts showed no difference in wound healing when compared with the negative control group. The crude extract from leaves (1200 mg/kg) has equal efficacy in reducing temperature in rats with hyperpyrexia compared to dipyrone (240 mg/kg) and is better than paracetamol (150 mg/kg). In acute toxicity test, crude extract of leaves from Lippia thymoides exhibited no mortality and behavioral changes and no adverse effects in male and female mice. This work validates the popular use of Lippia thymoides for treating the wound and fever, providing a source for biologically active substances.
本研究旨在评估百里香叶下珠叶和茎的粗提物及其馏分，并验证其在民间医学中的应用。进行了体外抗氧化和抗菌活性以及大鼠体内伤口愈合、幼鼠面包酵母诱导发热和小鼠急性经口毒性试验。粗提物及其二氯甲烷和乙酸乙酯馏分对DPPH具有较强的自由基清除活性，但在β-胡萝卜素漂白法中无效。叶提取物的二氯甲烷馏分对金黄色葡萄球菌、蜡样芽孢杆菌和近平滑念珠菌表现出最广泛的活性谱。与阴性对照组相比，用粗提物处理的动物在伤口愈合方面没有差异。叶粗提物（1200mg/kg）在降低高热大鼠体温方面与安乃近（240mg/kg）疗效相当，且优于对乙酰氨基酚（150mg/kg）。在急性毒性试验中，百里香叶粗提物对雄性和雌性小鼠均未表现出死亡、行为变化及不良反应。本研究验证了百里香叶下珠在治疗伤口和发热方面的广泛应用，为生物活性物质提供了来源。
