Shi Lingyan, Rodríguez-Contreras Adrián, Budansky Yury, Pu Yang, Nguyen Thien An, Alfano Robert R
The City College of the City University of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York 10031bThe City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Departments of Electrical E.
The City College of the City University of New York, Department of Biology, 160 Convent Avenue, New York 10031.
J Biomed Opt. 2014 Jun;19(6):066009. doi: 10.1117/1.JBO.19.6.066009.
Two-photon (2P) excitation of the second singlet (S₂) state was studied to achieve deep optical microscopic imaging in brain tissue when both the excitation (800 nm) and emission (685 nm) wavelengths lie in the "tissue optical window" (650 to 950 nm). S₂ state technique was used to investigate chlorophyll α (Chl α) fluorescence inside a spinach leaf under a thick layer of freshly sliced rat brain tissue in combination with 2P microscopic imaging. Strong emission at the peak wavelength of 685 nm under the 2P S₂ state of Chl α enabled the imaging depth up to 450 μm through rat brain tissue.
当激发波长（800纳米）和发射波长（685纳米）都位于“组织光学窗口”（650至950纳米）内时，研究了对第二单线态（S₂）的双光子（2P）激发，以实现脑组织中的深度光学显微成像。结合2P显微成像技术，利用S₂态技术研究了新鲜切片大鼠脑组织厚层下菠菜叶内叶绿素α（Chlα）的荧光。在Chlα的2P S₂态下，685纳米峰值波长处的强发射使得能够穿透大鼠脑组织进行高达450微米的成像深度。
