嗜酸菌属CHX100中的新型环己烷单加氧酶。
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100.
作者信息
Salamanca Diego, Karande Rohan, Schmid Andreas, Dobslaw Daniel
机构信息
Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany.
出版信息
Appl Microbiol Biotechnol. 2015 Aug;99(16):6889-97. doi: 10.1007/s00253-015-6599-9. Epub 2015 May 3.
Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols.
嗜酸菌属CHX100菌株具有非凡的能力，能够在有氧条件下，通过一种未知机制，以短链环烷烃（C5 - C8）作为唯一碳源和能源进行生长。对嗜酸菌属CHX100菌株进行转座子诱变，发现了一种新型细胞色素P450单加氧酶（CYP450chx），它催化环己烷转化为环己醇。考虑到其操纵子结构：单加氧酶、FAD氧化还原酶和铁氧化还原蛋白，引物步移法将CYP450chx归类为细胞色素P450 I类。CYP450chx已成功克隆并在大肠杆菌JM109中表达。通过吲哚共氧化证明了CYP450chx的活性。通过将环烷烃（C5 - C8）催化转化为各自的环醇，证实了CYP450chx的生物转化能力。
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