Salamanca Diego, Karande Rohan, Schmid Andreas, Dobslaw Daniel
Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany.
Appl Microbiol Biotechnol. 2015 Aug;99(16):6889-97. doi: 10.1007/s00253-015-6599-9. Epub 2015 May 3.
Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols.
嗜酸菌属CHX100菌株具有非凡的能力，能够在有氧条件下，通过一种未知机制，以短链环烷烃（C5 - C8）作为唯一碳源和能源进行生长。对嗜酸菌属CHX100菌株进行转座子诱变，发现了一种新型细胞色素P450单加氧酶（CYP450chx），它催化环己烷转化为环己醇。考虑到其操纵子结构：单加氧酶、FAD氧化还原酶和铁氧化还原蛋白，引物步移法将CYP450chx归类为细胞色素P450 I类。CYP450chx已成功克隆并在大肠杆菌JM109中表达。通过吲哚共氧化证明了CYP450chx的活性。通过将环烷烃（C5 - C8）催化转化为各自的环醇，证实了CYP450chx的生物转化能力。
