Wang Rui, Zhang Ju-Liang, Wei Bo, Tian Yu, Li Zhao-Hui, Wang Le, Du Chao
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, 130033, China.
Tumour Biol. 2014 Oct;35(10):9661-6. doi: 10.1007/s13277-014-2211-7. Epub 2014 Jun 27.
The immune system fails to eradicate established tumors partly due to the induction of immune tolerance within tumor microenvironment. Plasmacytoid dendritic cells (pDCs) play critical roles in regulating immune system. In this study, we investigated pDC in the peripheral blood of glioma. CD4 + CD123 + BDCA2+ pDCs were tested from peripheral blood mononuclear cells in 40 glioma patients and 40 healthy controls by flow cytometry. The results revealed that proportion of pDCs was significantly increased in cases than in controls (0.52 ± 0.07 versus 0.21 ± 0.02 %, p < 0.001), whereas myeloid dendritic cells (mDCs) did not present any obvious difference between patients and healthy donors (0.25 ± 0.04 versus 0.18 ± 0.02 %, p = 0.217). We further studied pDCs in glioma patients with different clinical stages. Data showed that cases with smoking history had elevated level of pDCs than those non-smoker patients (0.91 ± 0.16 versus 0.48 ± 0.06 %, p = 0.004). Interestingly, we observed that patients with aphasia presented significantly elevated pDCs than those without aphasia (0.93 ± 0.12 versus 0.41 ± 0.07 %, p < 0.001). These data suggested that pDCs may be closely involved in the pathogenesis of glioma and may play roles in certain symptoms of the disease.
免疫系统无法根除已形成的肿瘤，部分原因是肿瘤微环境中诱导了免疫耐受。浆细胞样树突状细胞（pDCs）在调节免疫系统中起关键作用。在本研究中，我们调查了神经胶质瘤患者外周血中的pDC。通过流式细胞术检测了40例神经胶质瘤患者和40例健康对照者外周血单个核细胞中的CD4 + CD123 + BDCA2 + pDC。结果显示，病例组中pDC的比例显著高于对照组（0.52±0.07对0.21±0.02%，p<0.001），而髓样树突状细胞（mDCs）在患者和健康供体之间没有明显差异（0.25±0.04对0.18±0.02%，p = 0.217）。我们进一步研究了不同临床分期的神经胶质瘤患者中的pDC。数据显示，有吸烟史的病例中pDC水平高于无吸烟史的患者（0.91±0.16对0.48±0.06%，p = 0.004）。有趣的是，我们观察到失语患者的pDC明显高于无失语患者（0.93±0.12对0.41±0.07%，p<0.001）。这些数据表明，pDC可能密切参与神经胶质瘤的发病机制，并可能在该疾病的某些症状中起作用。
