Cao Jiangnan, Liu Xiaoqiu, Yao Huizhen, Yang Haibin, Wang Xiaolong, Sun Wenling
Department of Prosthodontics, School of Stomatology, Jilin University, Changchun 130021, China.
Department of Prosthodontics, School of Stomatology, Jilin University, Changchun 130021, China. Email:
Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Apr;49(4):229-33.
To investigate celluar toxicity and antibacterial property of nano-silver coating modified denture base and to provide a theortical basis for clinical application.
The samples were divided into coating group and denture base group. Samples in coating groups were treated with silver ion sputtering and were further divided into the 10, 20, 40, 60, 80, 100 s subgroups according to their sputtering time. Surface microstructure of samples in coating groups and denture base group were observed by scanning electron microscopy (SEM), and composition of material surface were determined with energy dispersive spectrum (EDS) analysis. Methyl thiazolyl terazolium (MTT) method was used to analysis L929 cells proliferation rate and cell toxicity grade while the coating groups (n = 6) and group test specimens (n = 6), negative control (polyethylene, PE) (n = 6) and positive control group (PVC-org.Sn) (n = 6) were co-cultured with L929 cells. The antibacterial properties of coating groups were investigated by using the bacterial membrane adhering method with Streptococcus mutans and Candida albicans.
SEM observation showed that the surface of control group specimens were smooth, whereas dense and uniform distributed nano silver particles were visible on the surface of coating group specimens. The silver particles were round, and the diameter size was 10 nm. MTT assay showed that after being co-cultured with specimens in control group, L929 cell had higher proliferation rate than 100% at 24, 48, 72 h, and cytotoxicity were graded 0. The membrane adhering method results showed that all of bacterial colonies were found in control group, but without anything at the coating groups.
Deposition of nano silver on the denture base surface by ion sputtering method is a viable method. There's no cytotoxicity of series Ag-coating material. Nano-silver modified denture base material benefits L929 cell surface proliferation and has antibacterial action.
研究纳米银涂层改性义齿基托的细胞毒性及抗菌性能，为其临床应用提供理论依据。
将样本分为涂层组和义齿基托组。涂层组样本采用银离子溅射处理，并根据溅射时间进一步分为10、20、40、60、80、100 s亚组。通过扫描电子显微镜（SEM）观察涂层组和义齿基托组样本的表面微观结构，并用能谱（EDS）分析确定材料表面成分。采用甲基噻唑基四唑（MTT）法分析涂层组（n = 6）、测试标本组（n = 6）、阴性对照组（聚乙烯，PE）（n = 6）和阳性对照组（PVC-有机锡）（n = 6）与L929细胞共培养时L929细胞的增殖率及细胞毒性等级。采用变形链球菌和白色念珠菌的细菌膜黏附法研究涂层组的抗菌性能。
SEM观察显示，对照组样本表面光滑，而涂层组样本表面可见致密且均匀分布的纳米银颗粒。银颗粒呈圆形，直径大小为10 nm。MTT分析显示，与对照组样本共培养后，L929细胞在24、48、72 h时增殖率高于100%，细胞毒性分级为0。膜黏附法结果显示，对照组均有细菌菌落生长，而涂层组未发现任何细菌菌落。
通过离子溅射法在义齿基托表面沉积纳米银是一种可行的方法。系列银涂层材料无细胞毒性。纳米银改性义齿基托材料有利于L929细胞表面增殖且具有抗菌作用。
