Visiting Fellow, Department of Pediatric Dentistry and Community Oral Health, University of Tennessee Health Science Center, Memphis, Tenn.
Research Associate, Department of Pediatric Dentistry and Community Oral Health, University of Tennessee Health Science Center, Memphis, Tenn.
J Prosthet Dent. 2017 Dec;118(6):765-770. doi: 10.1016/j.prosdent.2017.01.003. Epub 2017 Apr 21.
Candida-associated denture stomatitis is the most common oral mucosal lesion among denture wearers. Trimethylsilane (TMS) plasma coating may inhibit the growth of Candida albicans on denture surfaces.
The purpose of this in vitro study was to investigate whether TMS plasma coatings can effectively reduce C albicans adhesion on denture base acrylic resin surfaces.
Sixty denture base acrylic resin disks with smooth and rough surfaces were prepared and were either left untreated (control group) or coated with TMS monomer (experimental group) by using plasma. Contact angles were measured immediately after TMS plasma coating. The morphology of C albicans adhesion was observed with scanning electron microscopy (SEM). Energy-dispersive spectroscopy (EDS) was used to characterize the elemental composition of the specimen surface. An adhesion test was performed by incubating the resin disk specimens in C albicans suspensions (1×10 cells/mL) at 37°C for 24 hours and further measuring the optical density of the C albicans by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay test. One-way ANOVA and 2-way ANOVA were followed by a post hoc test analysis (α=.05).
The group with TMS coating exhibited a more hydrophobic surface than the control group. EDS analysis revealed successful TMS plasma coating. The difference in the mean contact angles between the uncoated group and the TMS-coated group was statistically significant (P<.05), 79.0 ±2.9 degrees versus 105.7 ±1.5 degrees for the smooth surface and 90.2 ±7.6 degrees versus 131.5 ±2.1 degrees for the rough surface. In SEM analysis, the C albicans biofilm was found to grow more on the surface of the denture base resin without the TMS coating than on the surfaces of the experimental group. In the adhesion test, the amount of C albicans adhering to the surface of denture base resin with the TMS coating was significantly less than that on the surfaces without TMS coating (P<.05).
TMS coating significantly reduced the adhesion of C albicans to the denture base resin and may reduce denture stomatitis.
念珠菌相关性义齿性口炎是义齿佩戴者中最常见的口腔黏膜病变。三甲基硅烷（TMS）等离子体涂层可能抑制白色念珠菌在义齿表面的生长。
本体外研究的目的是探讨 TMS 等离子体涂层是否能有效减少白色念珠菌在义齿基托丙烯酸树脂表面的黏附。
制备 60 个具有光滑和粗糙表面的义齿基托丙烯酸树脂圆盘，分为未处理（对照组）和 TMS 单体等离子体涂层（实验组）。TMS 等离子体涂层后立即测量接触角。扫描电子显微镜（SEM）观察白色念珠菌黏附的形态。能谱仪（EDS）用于表征样品表面的元素组成。将树脂盘标本在白色念珠菌悬浮液（1×10 个细胞/ml）中于 37°C孵育 24 小时，然后通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定试验进一步测量白色念珠菌的光密度，进行黏附试验。采用单因素方差分析和双因素方差分析，然后进行事后检验分析（α=.05）。
TMS 涂层组的表面表现出比对照组更疏水的表面。EDS 分析表明 TMS 等离子体涂层成功。未涂层组和 TMS 涂层组的平均接触角差异有统计学意义（P<.05），光滑表面分别为 79.0±2.9 度和 105.7±1.5 度，粗糙表面分别为 90.2±7.6 度和 131.5±2.1 度。在 SEM 分析中，发现白色念珠菌生物膜在未涂覆 TMS 的义齿基托树脂表面上的生长比在实验组的表面上更多。在黏附试验中，TMS 涂层表面的义齿基托树脂黏附的白色念珠菌数量明显少于未涂覆 TMS 涂层的表面（P<.05）。
TMS 涂层显著减少了白色念珠菌对义齿基托树脂的黏附，可能减少义齿性口炎。
