Wilches Ricardo, Voigt Susanne, Duchen Pablo, Laurent Stefan, Stephan Wolfgang
Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany
Section of Evolutionary Biology, Department of Biology II, Ludwig-Maximilian University of Munich, 82152 Planegg-Martinsried, Germany.
G3 (Bethesda). 2014 Jun 26;4(9):1635-45. doi: 10.1534/g3.114.012757.
There is a growing interest in investigating the relationship between genes with signatures of natural selection and genes identified in QTL mapping studies using combined population and quantitative genetics approaches. We dissected an X-linked interval of 6.2 Mb, which contains two QTL underlying variation in chill coma recovery time (CCRT) in Drosophila melanogaster from temperate (European) and tropical (African) regions. This resulted in two relatively small regions of 131 kb and 124 kb. The latter one co-localizes with a very strong selective sweep in the European population. We examined the genes within and near the sweep region individually using gene expression analysis and P-element insertion lines. Of the genes overlapping with the sweep, none appears to be related to CCRT. However, we have identified a new candidate gene of CCRT, brinker, which is located just outside the sweep region and is inducible by cold stress. We discuss these results in light of recent population genetics theories on quantitative traits.
利用群体遗传学和数量遗传学相结合的方法，研究具有自然选择特征的基因与数量性状位点（QTL）定位研究中鉴定出的基因之间的关系，正受到越来越多的关注。我们剖析了一个6.2 Mb的X连锁区间，该区间包含两个QTL，它们是温带（欧洲）和热带（非洲）地区黑腹果蝇冷昏迷恢复时间（CCRT）变异的基础。这产生了两个相对较小的区域，分别为131 kb和124 kb。后者与欧洲群体中一个非常强烈的选择性清除区域共定位。我们使用基因表达分析和P元素插入系分别检查了清除区域内和附近的基因。与清除区域重叠的基因中，没有一个似乎与CCRT相关。然而，我们已经鉴定出一个新的CCRT候选基因——brinker，它位于清除区域之外，并且可被冷应激诱导。我们根据最近关于数量性状的群体遗传学理论讨论了这些结果。
