Wu Zhiqiang
Department of Gastroenterology, First Affiliated Hospital, People's Liberation Army General Hospital, Beijing 100037, China.
J Biol Chem. 2014 Aug 15;289(33):22630-22635. doi: 10.1074/jbc.M114.569459. Epub 2014 Jun 26.
The immune regulatory functions of B cells are not fully understood yet. The present study aims to characterize a subtype of B cells that expresses CX3CR1. In this study, peripheral blood samples were collected from patients with food allergies and healthy subjects. Peripheral B cells were analyzed by flow cytometry. T cell proliferation was assessed by carboxyfluorescein succinimidyl ester dilution assay. The results showed that the CX3CR1(+) B cells were detected in the peripheral blood samples of healthy subjects and were significantly less in patients with food allergies. CX3CR1(+) B cells expressed high levels of TGF-β and integrin αvβ6. CX3CR1(+) B cells could efficiently suppress other effector CD4(+) T cell activation. We conclude that human peripheral CX3CR1(+) B cells have immune suppressor properties.
B细胞的免疫调节功能尚未完全明确。本研究旨在鉴定表达CX3CR1的B细胞亚型。在本研究中，采集了食物过敏患者和健康受试者的外周血样本。通过流式细胞术分析外周B细胞。采用羧基荧光素琥珀酰亚胺酯稀释法评估T细胞增殖。结果显示，健康受试者外周血样本中可检测到CX3CR1(+) B细胞，而食物过敏患者的CX3CR1(+) B细胞明显较少。CX3CR1(+) B细胞高表达TGF-β和整合素αvβ6。CX3CR1(+) B细胞能够有效抑制其他效应性CD4(+) T细胞的激活。我们得出结论，人外周CX3CR1(+) B细胞具有免疫抑制特性。
