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群体分布分析揭示了平行内吞途径背后分子参与者的层级结构。

Population distribution analyses reveal a hierarchy of molecular players underlying parallel endocytic pathways.

作者信息

Gupta Gagan D, Dey Gautam, Swetha M G, Ramalingam Balaji, Shameer Khader, Thottacherry Joseph Jose, Kalappurakkal Joseph Mathew, Howes Mark T, Chandran Ruma, Das Anupam, Menon Sindhu, Parton Robert G, Sowdhamini R, Thattai Mukund, Mayor Satyajit

机构信息

National Centre for Biological Sciences, Tata Institute of Fundamental Research, UAS/GKVK Campus, Bangalore, India.

The University of Queensland, Institute for Molecular Bioscience, Queensland, Australia.

出版信息

PLoS One. 2014 Jun 27;9(6):e100554. doi: 10.1371/journal.pone.0100554. eCollection 2014.

DOI:10.1371/journal.pone.0100554
PMID:24971745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4074053/
Abstract

Single-cell-resolved measurements reveal heterogeneous distributions of clathrin-dependent (CD) and -independent (CLIC/GEEC: CG) endocytic activity in Drosophila cell populations. dsRNA-mediated knockdown of core versus peripheral endocytic machinery induces strong changes in the mean, or subtle changes in the shapes of these distributions, respectively. By quantifying these subtle shape changes for 27 single-cell features which report on endocytic activity and cell morphology, we organize 1072 Drosophila genes into a tree-like hierarchy. We find that tree nodes contain gene sets enriched in functional classes and protein complexes, providing a portrait of core and peripheral control of CD and CG endocytosis. For 470 genes we obtain additional features from separate assays and classify them into early- or late-acting genes of the endocytic pathways. Detailed analyses of specific genes at intermediate levels of the tree suggest that Vacuolar ATPase and lysosomal genes involved in vacuolar biogenesis play an evolutionarily conserved role in CG endocytosis.

摘要

单细胞分辨率测量揭示了果蝇细胞群体中网格蛋白依赖性（CD）和非依赖性（CLIC/GEEC：CG）内吞活性的异质分布。dsRNA介导的核心与外周内吞机制的敲低分别导致这些分布的平均值发生强烈变化或形状发生细微变化。通过量化27个报告内吞活性和细胞形态的单细胞特征的这些细微形状变化，我们将1072个果蝇基因组织成一个树状层次结构。我们发现树节点包含富含功能类别和蛋白质复合物的基因集，提供了CD和CG内吞作用的核心和外周控制的概况。对于470个基因，我们从单独的测定中获得了额外的特征，并将它们分类为内吞途径的早期或晚期作用基因。对树中间水平的特定基因的详细分析表明，参与液泡生物发生的液泡ATP酶和溶酶体基因在CG内吞作用中发挥着进化保守的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/d3b6715c2a3a/pone.0100554.g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/de1136f06f69/pone.0100554.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/3f6f1a4d8ab3/pone.0100554.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/d3b6715c2a3a/pone.0100554.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/315228b84e7d/pone.0100554.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/faf67edd0218/pone.0100554.g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcf5/4074053/d3b6715c2a3a/pone.0100554.g008.jpg

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