Varona-Uribe Marcela Eugenia, Torres-Rey Carlos H, Díaz-Criollo Sonia, Palma-Parra Ruth Marien, Narváez Diana María, Carmona Sandra Patricia, Briceño Leonardo, Idrovo Alvaro J
a Departamento de Salud Pública, Universidad del Rosario , Bogotá , DC , Colombia.
b Grupo de Salud Ocupacional y del Ambiente, Instituto Nacional de Salud , Bogotá , DC , Colombia.
Arch Environ Occup Health. 2016;71(1):3-9. doi: 10.1080/19338244.2014.910489. Epub 2014 Jun 27.
This study describes the use of pesticides mixtures and their potential association with comet assay results in 223 rice field workers in Colombia. Thirty-one pesticides were quantified in blood, serum, and urine (15 organochlorines, 10 organophosphorus, 5 carbamates, and ethylenethiourea), and the comet assay was performed. Twenty-four (77.42%) pesticides were present in the workers. The use of the maximum-likelihood factor analysis identified 8 different mixtures. Afterwards, robust regressions were used to explore associations between the factors identified and the comet assay. Two groups of mixtures--α-benzene hexachloride (α-BHC), hexachlorobenzene (HCB), and β-BHC (β: 1.21, 95% confidence interval [CI]: 0.33-2.10) and pirimiphos-methyl, malathion, bromophos-methyl, and bromophos-ethyl (β: 11.97, 95% CI: 2.34-21.60)--were associated with a higher percentage of DNA damage and comet tail length, respectively. The findings suggest that exposure to pesticides varies greatly among rice field workers.
本研究描述了哥伦比亚223名稻田工人使用农药混合物的情况及其与彗星试验结果的潜在关联。对血液、血清和尿液中的31种农药（15种有机氯农药、10种有机磷农药、5种氨基甲酸酯类农药和乙撑硫脲）进行了定量分析，并进行了彗星试验。在这些工人中检测到了24种（77.42%）农药。使用最大似然因子分析确定了8种不同的混合物。随后，采用稳健回归来探究所确定的因子与彗星试验之间的关联。两组混合物——α-六六六（α-BHC）、六氯苯（HCB）和β-六六六（β：1.21，95%置信区间[CI]：0.33 - 2.10）以及甲基嘧啶磷、马拉硫磷、甲基溴磷和乙基溴磷（β：11.97，95% CI：2.34 - 21.60）——分别与更高比例的DNA损伤和彗星尾长相关。研究结果表明，稻田工人接触农药的情况差异很大。
