Jin Hongli, Liu Yanfang, Guo Zhimou, Wang Jixia, Zhang Xiuli, Wang Chaoran, Liang Xinmiao
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People's Republic of China.
J Pharm Biomed Anal. 2016 Oct 25;130:336-346. doi: 10.1016/j.jpba.2016.06.008. Epub 2016 Jun 7.
Traditional Chinese Medicine (TCM) is an ancient medical practice which has been used to prevent and cure diseases for thousands of years. TCMs are frequently multi-component systems with mainly unidentified constituents. The study of the chemical compositions of TCMs remains a hotspot of research. Different strategies have been developed to manage the significant complexity of TCMs, in an attempt to determine their constituents. Reversed-phase liquid chromatography (RPLC) is still the method of choice for the separation of TCMs, but has many problems related to limited selectivity. Recently, enormous efforts have been concentrated on the development of efficient liquid chromatography (LC) methods for TCMs, based on selective stationary phases. This can improve the resolution and peak capacity considerably. In addition, high-efficiency stationary phases have been applied in the analysis of TCMs since the invention of ultra high-performance liquid chromatography (UHPLC). This review describes the advances in LC methods in TCM research from 2010 to date, and focuses on novel stationary phases. Their potential in the separation of TCMs using relevant applications is also demonstrated.
传统中医（TCM）是一种古老的医学实践，数千年来一直用于预防和治疗疾病。中药通常是多成分体系，其成分大多不明。对中药化学成分的研究仍然是一个研究热点。为了应对中药显著的复杂性，人们已开发出不同策略来确定其成分。反相液相色谱（RPLC）仍是分离中药的首选方法，但存在选择性有限等诸多问题。近年来，基于选择性固定相，人们投入了大量精力开发高效液相色谱（LC）方法用于中药分析。这可显著提高分离度和峰容量。此外，自超高效液相色谱（UHPLC）发明以来，高效固定相已应用于中药分析。本文综述了2010年至今液相色谱方法在中药研究中的进展，并重点介绍了新型固定相。还展示了它们在相关应用中用于分离中药的潜力。
