Department of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
Rejuvenation Res. 2013 Aug;16(4):295-303. doi: 10.1089/rej.2013.1420.
Trophocytes and fat cells of queen honeybees have been used for delayed cellular senescence studies, but their oxidative stress and anti-oxidant enzyme activities with advancing age are unknown. In this study, we assayed reactive oxygen species (ROS) and anti-oxidant enzymes in the trophocytes and fat cells of young and old queens. Young queens had lower ROS levels, lower superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and higher thioredoxin reductase (TR) activity compared to old queens. These results show that oxidative stress and anti-oxidant enzyme activities in trophocytes and fat cells increase with advancing age in queens and suggest that an increase in oxidative stress and a consequent increase in stress defense mechanisms are associated with the longevity of queen honeybees.
蜂王的滋养细胞和脂肪细胞已被用于研究细胞衰老的延迟，但它们的氧化应激和抗氧化酶活性随年龄的增长而变化尚不清楚。在这项研究中，我们检测了年轻和年老蜂王的滋养细胞和脂肪细胞中的活性氧（ROS）和抗氧化酶。与年老蜂王相比，年轻蜂王的 ROS 水平较低，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性较低，而硫氧还蛋白还原酶（TR）活性较高。这些结果表明，蜂王的滋养细胞和脂肪细胞中的氧化应激和抗氧化酶活性随着年龄的增长而增加，这表明氧化应激的增加以及随之而来的应激防御机制的增加与蜂王的长寿有关。
