Svehlová Andrea, Berthová Lenka, Sallay Balázs, Boldiš Vojtech, Sparagano Olivier A E, Spitalská Eva
Institute of Virology, Slovak Academy of Sciences, Dúbravska cesta 9, 845 05 Bratislava, Slovak Republic.
HPL (Ltd) Medical Laboratories, Department of Parasitology, Istrijská 20, 841 07 Bratislava, Slovak Republic.
Ticks Tick Borne Dis. 2014 Sep;5(5):600-5. doi: 10.1016/j.ttbdis.2014.04.010. Epub 2014 Jun 24.
Vojka nad Dunajom in the south-west of the Slovak Republic is a locality with sympatric occurrence of 3 species of ticks. This study investigated the spatial distribution of Dermacentor reticulatus, Ixodes ricinus, and Haemaphysalis concinna ticks in this area and determined the prevalence of Babesia and Rickettsia species in questing adults of these tick species considered as potential risk for humans and animals. Ticks were collected by blanket dragging over the vegetation from September 2011 to October 2012. All ticks were subjected to DNA extraction and individually assayed with PCR-based methods targeting the gltA, sca4, 23S rRNA genes of Rickettsia spp. and the 18S rRNA gene of Babesia spp. D. reticulatus was the dominant species occurring in this area (67.7%, n=600), followed by I. ricinus (31.8%, n=282) and H. concinna (0.5%, n=4) ticks. Rickettsial infection was determined in 10.8% (n=65) and 11.7% (n=33) of D. reticulatus and I. ricinus ticks, respectively. Babesia spp. infection was confirmed in 1.8% (n=11) of D. reticulatus and 0.4% (n=1) of I. ricinus ticks. DNA of 6 different pathogenic tick-borne species, Rickettsia helvetica, Rickettsia monacensis, Rickettsia slovaca, Rickettsia raoultii, Babesia canis, and Babesia venatorum were identified in this locality with sympatric occurrence of I. ricinus, D. reticulatus, and H. concinna ticks.
位于斯洛伐克共和国西南部的多瑙河沿岸的沃伊卡是一个有三种蜱虫同域分布的地区。本研究调查了该地区网纹革蜱、蓖麻硬蜱和康氏血蜱的空间分布，并确定了这些蜱虫若虫中作为人和动物潜在风险的巴贝斯虫属和立克次氏体属物种的流行情况。2011年9月至2012年10月，通过在植被上用毯子拖拽的方式收集蜱虫。所有蜱虫都进行了DNA提取，并分别采用基于PCR的方法进行检测，这些方法针对立克次氏体属的gltA、sca4、23S rRNA基因以及巴贝斯虫属的18S rRNA基因。网纹革蜱是该地区的优势物种（67.7%，n = 600），其次是蓖麻硬蜱（31.8%，n = 282）和康氏血蜱（0.5%，n = 4）。分别在10.8%（n = 65）的网纹革蜱和11.7%（n = 33）的蓖麻硬蜱中检测到立克次氏体感染。在1.8%（n = 11）的网纹革蜱和0.4%（n = 1）的蓖麻硬蜱中确认了巴贝斯虫属感染。在这个蓖麻硬蜱、网纹革蜱和康氏血蜱同域分布的地区，鉴定出了6种不同的致病性蜱传物种的DNA，即瑞士立克次氏体、蒙氏立克次氏体、斯洛伐克立克次氏体、拉乌尔立克次氏体、犬巴贝斯虫和 Venatorum 巴贝斯虫。
