Key Laboratory of Analytical Chemistry for Biology and Medicine, (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China.
Key Laboratory of Analytical Chemistry for Biology and Medicine, (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China.
Biosens Bioelectron. 2014 Dec 15;62:19-24. doi: 10.1016/j.bios.2014.06.022. Epub 2014 Jun 17.
A novel water-compatible fenitrothion imprinted polymer was prepared on Au nanoparticles (AuNPs) by click chemistry and reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization (RAFTPP). The RAFT chain-transfer agent was synthesized on the surface of AuNPs using click chemistry, then an imprinted polymer with hydrophilic polymer brushes was prepared on the RAFT chain-transfer agent modified AuNPs by RAFTPP, mediated by hydrophilic polyethylene glycol macromolecular cochain-transfer agent. The obtained molecularly imprinted material showed improved accessibility to fenitrothion and recognition property in water medium. When the material was immobilized on an ionic liquid functionalized graphene coated glassy carbon electrode for the electrochemical determination of fenitrothion, the resulting electrochemical sensor presented linear response in the range of 0.01-5 μM, with a sensitivity of 6.1 μA/μM mm(2). The low limit of detection was 8 nM (S/N=3). The sensor was successfully applied to the determination of real samples and the recovery for standard added was 95-108%.
一种新型的水相兼容的虫螨腈印迹聚合物通过点击化学和可逆加成-断裂链转移（RAFT）沉淀聚合（RAFTPP）在金纳米粒子（AuNPs）上制备。RAFT 链转移剂通过点击化学在 AuNPs 表面合成，然后通过 RAFTPP 在 RAFT 链转移剂修饰的 AuNPs 上制备具有亲水性聚合物刷的印迹聚合物，该反应由亲水性聚乙二醇大分子链转移剂介导。所得的分子印迹材料在水介质中对虫螨腈具有更好的可及性和识别性能。当将该材料固定在离子液体功能化石墨烯涂覆的玻碳电极上用于电化学测定虫螨腈时，所得电化学传感器在 0.01-5 μM 的范围内呈现线性响应，灵敏度为 6.1 μA/μM mm(2)。检测限低至 8 nM（S/N=3）。该传感器成功地应用于实际样品的测定，标准添加的回收率为 95-108%。
