Wu Ningli, Luo Zhimin, Ge Yanhui, Guo Pengqi, Du Kangli, Tang Weili, Du Wei, Zeng Aiguo, Chang Chun, Fu Qiang
School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
Department of Pharmacy, Xi'an First Hospital, Xi'an 710002, China.
J Pharm Anal. 2016 Jun;6(3):157-164. doi: 10.1016/j.jpha.2016.01.004. Epub 2016 Jan 26.
Surface molecularly imprinted polymers (SMIPs) for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological characteristics of the polymers were investigated by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), elemental analysis and nitrogen adsorption-desorption test. The obtained results showed that the SMIPs displayed great adsorption capacity (13.5 μg/mg), high recognition ability (the imprinted factor is 3.2) and good binding kinetics for ampicillin sodium. Finally, as solid phase extraction adsorbents, the SMIPs coupled with HPLC method were validated and applied for the enrichment, purification and determination of ampicillin sodium in real milk and blood samples. The averages of spiked accuracy ranged from 92.1% to 107.6%. The relative standard deviations of intra- and inter-day precisions were less than 4.6%. This study provides a new and promising method for enriching, extracting and determining ampicillin sodium in complex biological samples.
以硅胶为载体，采用表面分子印迹技术合成了用于选择性吸附氨苄西林钠的表面分子印迹聚合物（SMIPs）。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、元素分析和氮吸附-脱附试验研究了聚合物的物理和形态特征。所得结果表明，SMIPs对氨苄西林钠具有很大的吸附容量（13.5 μg/mg）、高识别能力（印迹因子为3.2）和良好的结合动力学。最后，作为固相萃取吸附剂，将SMIPs与高效液相色谱法联用，对实际牛奶和血液样品中的氨苄西林钠进行了富集、纯化和测定。加标回收率平均值在92.1%至107.6%之间。日内和日间精密度的相对标准偏差均小于4.6%。本研究为复杂生物样品中氨苄西林钠的富集、提取和测定提供了一种新的且有前景的方法。
