Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
1] Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA. [2] Department of Electrical and Computer Engineering, Duke University, Durham, North Carolina, USA.
Nat Neurosci. 2014 Aug;17(8):1130-6. doi: 10.1038/nn.3751. Epub 2014 Jun 29.
Transcranial magnetic stimulation (TMS) is a widely used, noninvasive method for stimulating nervous tissue, yet its mechanisms of effect are poorly understood. Here we report new methods for studying the influence of TMS on single neurons in the brain of alert non-human primates. We designed a TMS coil that focuses its effect near the tip of a recording electrode and recording electronics that enable direct acquisition of neuronal signals at the site of peak stimulus strength minimally perturbed by stimulation artifact in awake monkeys (Macaca mulatta). We recorded action potentials within ∼1 ms after 0.4-ms TMS pulses and observed changes in activity that differed significantly for active stimulation as compared with sham stimulation. This methodology is compatible with standard equipment in primate laboratories, allowing easy implementation. Application of these tools will facilitate the refinement of next generation TMS devices, experiments and treatment protocols.
经颅磁刺激（TMS）是一种广泛应用的、非侵入性的刺激神经组织的方法，但它的作用机制还不太清楚。在这里，我们报告了新的方法，用于研究 TMS 对清醒的非人类灵长类动物大脑中单神经元的影响。我们设计了一种 TMS 线圈，使其在靠近记录电极尖端的地方聚焦，并设计了记录电子设备，使我们能够在清醒猴子（猕猴）中以最小的刺激伪影干扰在峰值刺激强度处直接获取神经元信号。我们在 0.4ms 的 TMS 脉冲后约 1ms 记录动作电位，并观察到与假刺激相比，主动刺激的活性变化有显著差异。这种方法与灵长类动物实验室的标准设备兼容，易于实现。这些工具的应用将有助于改进下一代 TMS 设备、实验和治疗方案。
