Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling 712100, Shaanxi, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
Vet Microbiol. 2014 Aug 27;172(3-4):415-24. doi: 10.1016/j.vetmic.2014.06.006. Epub 2014 Jun 10.
Hepatitis E virus (HEV), the causative agent of hepatitis E, is classified into four major genotypes (1 to 4) and swine is the main natural reservoir for genotypes 3 and 4. In this study, a total of 106 bile samples from a slaughterhouse in the Shandong province of China were tested for the partial ORF2 gene of HEV by RT-nPCR to determine the virus genotypes, and two indirect ELISA were developed for the detection of swine HEV specific IgM and IgG antibodies in 980 serum samples from 24 farms, in order to investigate the seroprevalence. Thirty-two out of 106 (30.2%) bile samples were positive for HEV and a high degree of partial ORF2 sequence similarity (86.8-100%) was observed among 20 samples. The viral sequences belonged to genotype 4, subtypes 4a and 4d. One complete genome sequence of a subtype 4d HEV was further determined and characterized. The seroprevalence of HEV IgG and IgM antibodies was 100% (24/24) and 41.7% (10/24) for herds, and 66.4% (651/980) and 1.6% (16/980) for the individual pigs, respectively. These results suggested a high prevalence of genotype 4 of swine HEV infection both in swine farms and at the slaughterhouse in Shandong province, which further raise public-health concerns for zoonosis and pork safety.
戊型肝炎病毒（HEV）是戊型肝炎的病原体，分为 4 个主要基因型（1 到 4），猪是基因型 3 和 4 的主要天然宿主。本研究采用 RT-nPCR 检测山东省某屠宰场的 106 份胆汁样本的 HEV 部分 ORF2 基因，以确定病毒基因型，并开发了两种间接 ELISA 法，用于检测来自 24 个农场的 980 份血清样本中的猪 HEV 特异性 IgM 和 IgG 抗体，以调查血清流行率。106 份胆汁样本中有 32 份（30.2%）为 HEV 阳性，20 份样本的部分 ORF2 序列高度相似（86.8%到 100%）。病毒序列属于基因型 4，亚型 4a 和 4d。进一步确定并分析了一个亚型 4d HEV 的完整基因组序列。猪群的 HEV IgG 和 IgM 抗体血清流行率分别为 100%（24/24）和 41.7%（24/24），个体猪的分别为 66.4%（651/980）和 1.6%（16/980）。这些结果表明，山东省猪群和屠宰场存在高感染率的猪基因型 4 HEV，这进一步引起了人们对人畜共患病和猪肉安全的公共卫生关注。
