Department of Medical Genetics, Istanbul University, Cerrahpasa Medical School, Istanbul, Turkey; Department of Molecular Biology and Genetics, Biruni University, Istanbul, Turkey.
Int J Cancer. 2015 Feb 15;136(4):875-9. doi: 10.1002/ijc.29054. Epub 2014 Jul 7.
Prostate cancer (PCa) is one of the leading causes of cancer deaths in men. Since there are limited treatment options available for the advanced tumors, there is an urgent need for novel diagnostic tools for PCa. Prostate secretion samples (PSS) from 23 PCa and 25 benign prostate hyperplasia (BPH) patients were obtained from Urology Department of Bagcilar Educational and Research Hospital (Istanbul). MicroRNA (miRNA) profiling of eight PSS (four from BPH, four from PCa patients) was performed using microarray. Four of significantly deregulated miRNAs were further confirmed using quantitative reverse-transcription PCR (qRT-PCR). Statistical analysis was performed using Student's t-test. ROC curves were plotted with SPSS-15.0. In this study, we aimed to identify a miRNA expression signature that could be used to distinguish PCa from BPH. MiRNA profiling of four PCa and four BPH patients with microarray revealed that miR-361-3p, miR-133b and miR-221 were significantly downregulated and miR-203 was upregulated in PSS of PCa patients. Further qRT-PCR analysis confirmed the altered expressions of these four miRNAs in PSS of 23 PCa and 25 BPH patients. Four miRNAs, together and individually have much power (AUC; 0.950) than PSA has (AUC; 0.463) to discriminate PCa from BPH patients. We have shown for the first time in the literature the presence of miRNAs in the PSS. We suggest PSS as a powerful non-invasive source for evaluation of prognosis in PCa, since prostate massages can be easily applied during routine examination. Our results showed that certain differentially expressed miRNAs in PSS could be used as diagnostics markers.
前列腺癌（PCa）是男性癌症死亡的主要原因之一。由于晚期肿瘤的治疗选择有限，因此迫切需要新型的 PCa 诊断工具。本研究从 Bagcilar 教育和研究医院（伊斯坦布尔）泌尿科获得了 23 例 PCa 和 25 例良性前列腺增生（BPH）患者的前列腺分泌物样本（PSS）。使用微阵列对 8 个 PSS（4 个来自 BPH，4 个来自 PCa 患者）的 microRNA（miRNA）谱进行分析。使用定量逆转录 PCR（qRT-PCR）进一步验证了 4 个显著下调的 miRNA。使用 Student's t 检验进行统计分析。使用 SPSS-15.0 绘制 ROC 曲线。在这项研究中，我们旨在确定一种 miRNA 表达特征，可用于区分 PCa 和 BPH。微阵列分析显示，miR-361-3p、miR-133b 和 miR-221 在 PCa 患者的 PSS 中显著下调，miR-203 上调。进一步的 qRT-PCR 分析证实了这些 miRNA 在 23 例 PCa 和 25 例 BPH 患者 PSS 中的改变表达。这四个 miRNA 一起和单独具有比 PSA 更高的区分 PCa 和 BPH 患者的能力（AUC；0.950）。我们首次在文献中证明了 PSS 中存在 miRNAs。我们建议 PSS 作为评估 PCa 预后的有力非侵入性来源，因为在常规检查期间可以轻松进行前列腺按摩。我们的结果表明，PSS 中某些差异表达的 miRNA 可用作诊断标志物。
