Skripchenko A, Thompson-Montgomery D, Awatefe H, Turgeon A, Wagner S J
American Red Cross Biomedical Services, Holland Laboratory, Rockville, MD, USA.
Vox Sang. 2014 Nov;107(4):360-7. doi: 10.1111/vox.12174. Epub 2014 Jun 27.
Bacterial proliferation is inhibited in platelets (PLTs) stored at refrigerated temperatures, but also dramatically decreases PLT in vivo survival. Recent studies have demonstrated that cold temperature (CT) stored PLTs secrete sialidases upon re-warming, removing sialic acid from the PLT surface, which may be responsible for clustering of GPIbα and PLT clearance from circulation. In this study, the influence of a sialidase inhibitor or a p38 MAP kinase inhibitor was evaluated in units stored at 4 °C.
After collection of a single Trima apheresis unit (n = 12), PLTs were aliquoted into four 60-ml CLX storage bags. One bag was stored at 20-24 °C (RT) with continuous agitation; a second bag was stored at 4 °C without agitation; a third bag was held at 4 °C without agitation with sialidase inhibitor, a fourth bag was incubated at 4 °C with a p38 MAPK inhibitor without agitation.
Beginning from Day 1, all in vitro PLT parameters were adversely affected by CT compared to those of RT. Similar in vitro storage properties were observed in CT PLT in the presence or absence of sialidase or p38 MAPK inhibitors. P38 MAPK phosphorylation inhibition was not observed at CT. Decrease of sialidase activity was observed for 2 days in PLTs stored in additive solution but not in plasma.
Addition of either sialidase or p38 MAPK inhibitors do not improve any in vitro parameters of PLTs stored at 4 °C in 100% plasma.
冷藏温度下储存的血小板（PLT）中细菌增殖受到抑制，但也会显著降低PLT在体内的存活率。最近的研究表明，低温（CT）储存的PLT在复温时会分泌唾液酸酶，从PLT表面去除唾液酸，这可能是导致糖蛋白Ibα（GPIbα）聚集和PLT从循环中清除的原因。在本研究中，评估了唾液酸酶抑制剂或p38丝裂原活化蛋白激酶（MAPK）抑制剂对4℃储存的血小板单位的影响。
采集单个Trima血液成分分离机采集的单位血液（n = 12）后，将PLT分装到四个60 ml的CLX储存袋中。一个袋子在20 - 24℃（室温）下持续搅拌储存；第二个袋子在4℃下不搅拌储存；第三个袋子在4℃下不搅拌并加入唾液酸酶抑制剂；第四个袋子在4℃下加入p38 MAPK抑制剂并不搅拌孵育。
从第1天开始，与室温储存的PLT相比，所有体外PLT参数均受到低温的不利影响。在有或没有唾液酸酶或p38 MAPK抑制剂的情况下，低温储存的PLT具有相似的体外储存特性。在低温下未观察到p38 MAPK磷酸化受到抑制。在添加剂溶液中储存的PLT中，唾液酸酶活性在2天内下降，但在血浆中储存的PLT中未观察到这种情况。
添加唾液酸酶或p38 MAPK抑制剂并不能改善在100%血浆中于4℃储存的PLT的任何体外参数。
