Zhu Tie Pei, Tong Yu Hua, Zhan Hai Jing, Ma Jin
Eye Hospital of Wenzhou Medical University, No. 618 Fengqi East Road, Jianggan District, Hangzhou, Zhejiang Province, China.
Quzhou People's Hospital, Quzhou Central Hospital of Zhejiang Chinese Medical University, No. 2 Zhong Lou Di, Quzhou, Zhejiang Province, China.
Microvasc Res. 2014 Sep;95:7-14. doi: 10.1016/j.mvr.2014.06.007. Epub 2014 Jun 27.
This study was conducted to demonstrate a new scan method for retinal vessel structure measurement in a specific region of fundus (zone B) using spectral-domain optical coherence tomography (SD-OCT), and to assess its reliability. One temporal superior retinal vessel pair passing through a concentric ring (zone B), which was defined between half and one disc distance from the optic disc border, was chosen for the measurement using a volume scan in SD-OCT. On the SD-OCT image, retinal arteriolar outer diameter (RAOD), retinal arteriolar lumen diameter (RALD), retinal venular outer diameter (RVOD) and retinal venular lumen diameter (RVLD) were measured. Retinal vessel diameters on color fundus photographs were also analyzed. Fifty-five healthy individuals were recruited to evaluate intraobserver and interobserver reproducibility between the two examiners. The intraobserver intraclass correlation coefficient (ICC) ranged from 0.972 to 0.981, and the interobserver ICC ranged from 0.968 to 0.980. In the Bland-Altman plot, the 95% limits of interobserver agreement for the RAOD, RALD, RVOD and RVLD were -5.60 to 4.84μm, -5.78 to 5.05μm, -7.52 to 5.62μm and -7.10 to 5.63μm, respectively. The retinal arteriolar and venular lumen diameters on the SD-OCT image were close to the mean arteriolar and venular diameters obtained from the color fundus photographs. Volume scan method produced better images of retinal vessels showing the fine structures of the vessel wall, and provided reliable retinal vessel structure measurement in zone B with good repeatability and reproducibility.
本研究旨在展示一种使用光谱域光学相干断层扫描（SD - OCT）测量眼底特定区域（B区）视网膜血管结构的新扫描方法，并评估其可靠性。使用SD - OCT中的容积扫描，选择一对穿过同心环（B区）的颞侧上方视网膜血管，该同心环定义为距视盘边界半个至一个视盘直径的距离。在SD - OCT图像上，测量视网膜小动脉外径（RAOD）、视网膜小动脉管腔直径（RALD）、视网膜小静脉外径（RVOD）和视网膜小静脉管腔直径（RVLD）。还分析了彩色眼底照片上的视网膜血管直径。招募了55名健康个体来评估两位检查者之间的观察者内和观察者间的可重复性。观察者内组内相关系数（ICC）范围为0.972至0.981，观察者间ICC范围为0.968至0.980。在Bland - Altman图中，RAOD、RALD、RVOD和RVLD的观察者间一致性的95%界限分别为 - 5.60至4.84μm、 - 5.78至5.05μm、 - 7.52至5.62μm和 - 7.10至5.63μm。SD - OCT图像上的视网膜小动脉和小静脉管腔直径接近从彩色眼底照片获得的平均小动脉和小静脉直径。容积扫描方法产生了更好的视网膜血管图像，显示了血管壁的精细结构，并在B区提供了可靠的视网膜血管结构测量，具有良好的重复性和可重复性。
