Martinez-Pinna Roxana, Burillo Elena, Madrigal-Matute Julio, Lopez Juan Antonio, Camafeita Emilio, Torres-Fonseca Monica Maria, Llamas-Granda Patricia, Egido Jesus, Michel Jean-Baptiste, Blanco-Colio Luis Miguel, Martin-Ventura Jose Luis
Vascular Research Lab, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid.
Proteomics Clin Appl. 2014 Aug;8(7-8):626-30. doi: 10.1002/prca.201400035.
To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients.
RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot.
Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results.
RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.
检测腹主动脉瘤（AAA）患者的红细胞（RBC）膜成分是否发生改变。
采用基于无标记定量质谱的策略，利用谱图计数数据，对AAA患者（主动脉直径>3 cm，n = 7）和对照受试者（n = 4）的RBC膜提取物进行分析。通过蛋白质印迹法进行额外验证。
基于基于串联质谱实验的谱图计数的数据分析，为我们提供了39种在AAA患者和对照受试者的RBC膜中差异表达的蛋白质特征（变化等于/超过1.515倍；p值等于/低于0.05）。质谱数据显示，结构膜蛋白（如血影蛋白和锚蛋白）、降解机制成分（蛋白酶体亚基）以及氧化应激相关蛋白（如过氧化氢酶和过氧化物酶-2）等水平发生了改变。通过蛋白质印迹法进一步验证了与对照相比，AAA患者RBC膜中过氧化氢酶和过氧化物酶-2表达降低，证实了蛋白质组学结果。
AAA患者的RBC膜蛋白组成发生改变，这可能与RBC在主动脉组织中的病理作用有关，并成为预防AAA进展的潜在靶点。
