Tak Nisha, Gehlot Hukam S, Kaushik Muskan, Choudhary Sunil, Tiwari Ravi, Tian Rui, Hill Yvette, Bräu Lambert, Goodwin Lynne, Han James, Liolios Konstantinos, Huntemann Marcel, Palaniappan Krishna, Pati Amrita, Mavromatis Konstantinos, Ivanova Natalia, Markowitz Victor, Woyke Tanja, Kyrpides Nikos, Reeve Wayne
BNF and Stress Biology Lab, Department of Botany, JNV University, Jodhpur, India.
Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
Stand Genomic Sci. 2013 Dec 15;9(2):304-14. doi: 10.4056/sigs.4598281. eCollection 2013 Dec 20.
Ensifer sp. TW10 is a novel N2-fixing bacterium isolated from a root nodule of the perennial legume Tephrosia wallichii Graham (known locally as Biyani) found in the Great Indian (or Thar) desert, a large arid region in the northwestern part of the Indian subcontinent. Strain TW10 is a Gram-negative, rod shaped, aerobic, motile, non-spore forming, species of root nodule bacteria (RNB) that promiscuously nodulates legumes in Thar Desert alkaline soil. It is fast growing, acid-producing, and tolerates up to 2% NaCl and capable of growth at 40(o)C. In this report we describe for the first time the primary features of this Thar Desert soil saprophyte together with genome sequence information and annotation. The 6,802,256 bp genome has a GC content of 62% and is arranged into 57 scaffolds containing 6,470 protein-coding genes, 73 RNA genes and a single rRNA operon. This genome is one of 100 RNB genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
中华根瘤菌属TW10菌株是从印度次大陆西北部大干旱地区——印度大沙漠（当地称为比亚尼）的多年生豆科植物瓦氏灰叶豆（Tephrosia wallichii Graham）根瘤中分离得到的一种新型固氮细菌。TW10菌株是革兰氏阴性、杆状、需氧、具运动性、不产芽孢的根瘤菌（RNB），能在塔尔沙漠碱性土壤中与多种豆科植物形成根瘤。它生长迅速、产酸，能耐受高达2%的氯化钠，且能在40℃下生长。在本报告中，我们首次描述了这种塔尔沙漠土壤腐生菌的主要特征以及基因组序列信息和注释。该基因组大小为6,802,256 bp，GC含量为62%，由57个支架组成，包含6,470个蛋白质编码基因、73个RNA基因和一个rRNA操纵子。该基因组是美国能源部联合基因组研究所2010年细菌和古菌基因组百科全书——根瘤菌（GEBA-RNB）项目测序的100个根瘤菌基因组之一。
