Jaque Daniel, Rosal Blanca Del, Rodríguez Emma Martín, Maestro Laura Martínez, Haro-González Patricia, Solé José García
Fluorescence Imaging Group, Departamento de Física de Materiales, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Nanomedicine (Lond). 2014 May;9(7):1047-62. doi: 10.2217/nnm.14.59.
The importance of high-resolution intracellular thermal sensing and imaging in the field of modern biomedicine has boosted the development of novel nanosized fluorescent systems (fluorescent nanothermometers) as the next generation of probes for intracellular thermal sensing and imaging. This thermal mapping requires fluorescent nanothermometers with good biocompatibility and high thermal sensitivity in order to obtain submicrometric and subdegree spatial and thermal resolutions, respectively. This review describes the different nanosized systems used up to now for intracellular thermal sensing and imaging. We also include the later advances in molecular systems based on fluorescent proteins for thermal mapping. A critical overview of the state of the art and the future perspective is also included.
高分辨率细胞内热传感与成像在现代生物医学领域的重要性推动了新型纳米荧光系统（荧光纳米温度计）的发展，使其成为用于细胞内热传感与成像的下一代探针。这种热成像需要具有良好生物相容性和高热敏性的荧光纳米温度计，以便分别获得亚微米级和亚摄氏度级的空间和热分辨率。本综述描述了迄今为止用于细胞内热传感与成像的不同纳米系统。我们还纳入了基于荧光蛋白的分子系统在热成像方面的最新进展。此外，还对当前技术水平和未来前景进行了批判性概述。
