Khandelwal Nitin, Kaur Gurpreet, Chaubey Kundan Kumar, Singh Pushpendra, Sharma Shalini, Tiwari Archana, Singh Shoor Vir, Kumar Naveen
School of Biotechnology, Rajiv Gandhi Technical University, Airport Road, Bhopal, Madhya Pradesh 462036, India.
Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, Mathura, Uttar Pradesh 281122, India.
Virus Res. 2014 Sep 22;190:1-7. doi: 10.1016/j.virusres.2014.06.011. Epub 2014 Jun 28.
In the present study, we evaluated the antiviral efficacy of the silver nanoparticles (SNPs) against Peste des petits ruminants virus (PPRV), a prototype Morbillivirus. The leaf extract of the Argemone maxicana was used as a reducing agent for biological synthesis of the SNPs from silver nitrate. The SNPs were characterized using UV-vis absorption spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). The TEM analysis revealed particle size of 5-30 nm and the XRD analysis revealed their characteristic silver structure. The treatment of Vero cells with the SNPs at a noncytotoxic concentration significantly inhibited PPRV replication in vitro. The time-course and virus step-specific assays showed that the SNPs impair PPRV replication at the level of virus entry. The TEM analysis showed that the SNPs interact with the virion surface as well with the virion core. However, this interaction has no direct virucidal effect, instead exerts a blocking effect on viral entry into the target cells. This is the first documented evidence indicating that the SNPs are capable of inhibiting a Morbillivirus replication in vitro.
在本研究中，我们评估了银纳米颗粒（SNPs）对小反刍兽疫病毒（PPRV，一种麻疹病毒属原型病毒）的抗病毒效果。白屈菜的叶提取物被用作从硝酸银生物合成SNPs的还原剂。使用紫外可见吸收光谱、X射线衍射（XRD）和透射电子显微镜（TEM）对SNPs进行了表征。TEM分析显示颗粒大小为5 - 30纳米，XRD分析揭示了它们独特的银结构。用非细胞毒性浓度的SNPs处理Vero细胞可显著抑制PPRV在体外的复制。时间进程和病毒步骤特异性试验表明，SNPs在病毒进入水平损害PPRV复制。TEM分析表明，SNPs与病毒粒子表面以及病毒粒子核心相互作用。然而，这种相互作用没有直接的杀病毒作用，而是对病毒进入靶细胞产生阻断作用。这是首次有文献记载表明SNPs能够在体外抑制麻疹病毒属病毒复制的证据。
