Saadh Mohamed
Faculty of Pharmacy, Middle East University, Amman 11831, Jordan.
Faculty of Pharmacy, Philadelphia University , Amman, Jordan.
Saudi J Biol Sci. 2021 Nov;28(11):6674-6678. doi: 10.1016/j.sjbs.2021.07.035. Epub 2021 Jul 17.
Despite the fact that the Peste des petits ruminants virus (PPRV) leads to high morbidity and mortality (up to 100%), antiviral drugs against PPRV are not available. The aim of this study was to estimate the dose of epigallocatechin gallate (EGCG) co-administered with zinc (II) ions as an antiviral agent against PPRV. Treatment of PPRV-infectedVero cells with EGCG and zinc sulfate (zinc II) was administered, and antiviral activities against PPRV in infected Vero cells was evaluated by determination of virus yields, expressed as logTCID/mL. Cytotoxicity was determined using the tetrazolium-based MTS test. Zinc sulfate at 1.1 mg/mL and EGCG at 25 μM showed low potentiated and potentiated antiviral activities against PPRV, respectively. These agents caused significant inhibition of PPRV in Vero cells ( < 0.05) with a reduction in logTCID/mL by up to 3-fold. The combination of EGCG (25 μM) and zinc sulfate (1.1 mg/mL) was observed to have strong antiviral activity ( < 0.01) against PPRV with a reduction in logTCID/mL of the virus up to 4-times without causing any host cell cytotoxicity. This study is the first one to prove that the zinc II has the capability of stimulating EGCG to inhibit PPRV entry. Moreover, this combination appears capable of reducing infection resistance by hindering viral adaptation.
尽管小反刍兽疫病毒（PPRV）会导致高发病率和死亡率（高达100%），但目前尚无针对PPRV的抗病毒药物。本研究的目的是评估表没食子儿茶素没食子酸酯（EGCG）与锌（II）离子共同作为抗PPRV抗病毒剂的剂量。用EGCG和硫酸锌（锌II）处理感染PPRV的Vero细胞，并通过测定病毒产量（以logTCID/mL表示）来评估其对感染的Vero细胞中PPRV的抗病毒活性。使用基于四唑盐的MTS试验测定细胞毒性。1.1 mg/mL的硫酸锌和25 μM的EGCG分别对PPRV表现出低增强和增强的抗病毒活性。这些药物在Vero细胞中对PPRV有显著抑制作用（P<0.05），logTCID/mL降低高达3倍。观察到EGCG（25 μM）和硫酸锌（1.1 mg/mL）的组合对PPRV具有强大的抗病毒活性（P<0.01），病毒的logTCID/mL降低高达4倍，且不会引起任何宿主细胞毒性。本研究首次证明锌II有能力刺激EGCG抑制PPRV进入。此外，这种组合似乎能够通过阻碍病毒适应来降低感染抗性。
