Lee Changho, Jeon Mansik, Jeon Min Yong, Kim Jeehyun, Kim Chulhong
Appl Opt. 2014 Jun 20;53(18):3884-9. doi: 10.1364/AO.53.003884.
We have utilized a single pulsed broadband supercontinuum laser source to photoacoustically sense total hemoglobin concentration (HbT) and oxygen saturation of hemoglobin (SO2) in bloods in vitro. Unlike existing expensive and bulky laser systems typically used for functional photoacoustic imaging (PAI), our laser system is relatively cost-effective and compact. Instead of using two single wavelengths, two wavelength bands were applied to distinguish the concentrations of two different chromophores in the mixture. In addition, we have successfully extracted the total dye concentration and the ratio of the red dye concentration to the total dye concentration in mixed red and blue dye solutions in phantoms. The results indicate that PAI with a cheap and compact fiber based laser source can potentially provide HbT and SO2 in live animals in vivo.
我们利用单脉冲宽带超连续谱激光源对体外血液中的总血红蛋白浓度（HbT）和血红蛋白氧饱和度（SO2）进行光声传感。与通常用于功能光声成像（PAI）的现有昂贵且笨重的激光系统不同，我们的激光系统相对经济高效且紧凑。我们不是使用两个单一波长，而是应用两个波长带来区分混合物中两种不同发色团的浓度。此外，我们已成功提取了体模中混合红、蓝染料溶液的总染料浓度以及红色染料浓度与总染料浓度的比率。结果表明，使用基于光纤的廉价紧凑激光源的光声成像有潜力在活体动物体内提供HbT和SO2。
