Cardozo Viviane F, Lancheros Cesar A C, Narciso Adélia M, Valereto Elaine C S, Kobayashi Renata K T, Seabra Amedea B, Nakazato Gerson
Department of Microbiology, Universidade Estadual de Londrina, Campus Universitário - CEP 86055-990, Londrina, PR, Brazil.
Exact and Earth Sciences Department, Universidade Federal de São Paulo, Rua São Nicolau, 250 - CEP 09913-030, Diadema, SP, Brazil.
Int J Pharm. 2014 Oct 1;473(1-2):20-9. doi: 10.1016/j.ijpharm.2014.06.051. Epub 2014 Jun 27.
Bovine mastitis is a serious veterinary disease that causes great loss to the dairy industry worldwide. It is a major infectious disease and is difficult to manage and control. Furthermore, emerging multidrug resistant bacteria that cause mastitis have complicated such management. The free radical nitric oxide (NO) is a potent antimicrobial agent. Thus, the aims of this study were to prepare and evaluate the antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus (MBSA) and Escherichia coli (MBEC), which were isolated from bovine mastitis. Fifteen MBSA isolates and fifteen MBEC were collected from subclinical and clinical bovine mastitis. Biocompatible polymeric particles composed of alginate/chitosan or chitosan/sodium tripolyphosphate (TPP) were prepared and used to encapsulate mercaptosuccinic acid (MSA), which is a thiol-containing molecule. Nitrosation of thiol groups of MSA-containing particles formed S-nitroso-MSA particles, which are NO donors. The NO release kinetics from the S-nitroso-MSA particles showed sustained and controlled NO release over several hours. The antibacterial activity of NO-releasing particles was evaluated by incubating the particles with an MBSA multi-resistant strain, which is responsible for bovine mastitis. The minimum inhibitory concentration for S-nitroso-MSA-alginate/chitosan particles against MBSA ranged from 125 μg/mL to 250 μg/mL. The results indicate that NO-releasing polymeric particles are an interesting approach to combating bacteria resistance in bovine mastitis treatment and prevention.
牛乳腺炎是一种严重的兽医疾病，给全球乳制品行业造成巨大损失。它是一种主要的传染病，难以管理和控制。此外，引发乳腺炎的新型多重耐药细菌使这种管理变得更加复杂。自由基一氧化氮（NO）是一种有效的抗菌剂。因此，本研究的目的是制备并评估释放一氧化氮的聚合物颗粒对从牛乳腺炎中分离出的金黄色葡萄球菌（MBSA）和大肠杆菌（MBEC）的抗菌活性。从亚临床和临床牛乳腺炎中收集了15株MBSA分离株和15株MBEC。制备了由海藻酸盐/壳聚糖或壳聚糖/三聚磷酸钠（TPP）组成的生物相容性聚合物颗粒，并用于包封含硫醇的分子巯基琥珀酸（MSA）。含MSA颗粒的硫醇基团亚硝化形成了作为NO供体的S-亚硝基-MSA颗粒。S-亚硝基-MSA颗粒的NO释放动力学显示在数小时内NO持续且可控地释放。通过将颗粒与导致牛乳腺炎的MBSA多重耐药菌株一起孵育来评估释放NO颗粒的抗菌活性。S-亚硝基-MSA-海藻酸盐/壳聚糖颗粒对MBSA的最低抑菌浓度范围为125μg/mL至250μg/mL。结果表明，释放NO的聚合物颗粒是治疗和预防牛乳腺炎中对抗细菌耐药性的一种有趣方法。
