Nava Santiago, Barbieri Amalia M, Maya Leticia, Colina Rodney, Mangold Atilio J, Labruna Marcelo B, Venzal José M
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300 Rafaela, Santa Fe, Argentina.
Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Av. Prof. Orlando M. de Paiva 87, 05508-900 São Paulo, Brazil.
Acta Trop. 2014 Nov;139:1-4. doi: 10.1016/j.actatropica.2014.06.010. Epub 2014 Jun 28.
The aim of this work was to describe for the first time the presence of Borrelia burgdorferi sensu lato infecting ticks in Argentina. Unfed specimens of Ixodes pararicinus collected from vegetation in Jujuy Province were tested for Borrelia infection by PCR targeting the gene flagellin (fla), the rrfA-rrlB intergenic spacer region (IGS) and the 16S rDNA (rrs) gene. One male and one female of I. pararicinus collected in Jujuy were found to be positive to Borrelia infection with the three molecular markers tested. Phylogenetically, the Borrelia found in I. pararicinus from Jujuy belongs to the B. burgdorferi s.l complex, and it was similar to one of the genospecies detected in I. aragaoi from Uruguay. Also, this genospecies is closely related to two genospecies known from USA, Borrelia americana and the Borrelia sp. genospecies 1. The epidemiological risk that implies the infection with Borrelia in I. paracinus ticks from Argentina appears to be low because the genospecies detected is not suspected of having clinical relevance and there are no records of Ixodes ticks biting humans in the southern cone of South America. Further studies are needed to assess accurately if there is risk of borreliosis transmitted by ticks in South America.
这项工作的目的是首次描述在阿根廷感染蜱虫的伯氏疏螺旋体狭义种的存在情况。从胡胡伊省植被中采集的未进食的副瑞氏硬蜱标本，通过针对鞭毛蛋白（fla）基因、rrfA - rrlB基因间隔区（IGS）和16S rDNA（rrs）基因的PCR检测伯氏疏螺旋体感染情况。在胡胡伊采集的一只副瑞氏硬蜱雄性和一只雌性，经检测的三种分子标记均显示对伯氏疏螺旋体感染呈阳性。从系统发育来看，在胡胡伊的副瑞氏硬蜱中发现的伯氏疏螺旋体属于伯氏疏螺旋体狭义种复合体，并且与在乌拉圭的阿拉戈硬蜱中检测到的一种基因种相似。此外，这种基因种与在美国已知的两种基因种密切相关，即美洲伯氏疏螺旋体和伯氏疏螺旋体基因种1。阿根廷副瑞氏硬蜱感染伯氏疏螺旋体所隐含的流行病学风险似乎较低，因为检测到的基因种不被怀疑具有临床相关性，并且在南美洲南锥体地区没有硬蜱叮咬人类的记录。需要进一步研究以准确评估南美洲蜱虫传播莱姆病的风险。
