Saracho-Bottero María N, Tarragona Evelina L, Sebastian Patrick S, Venzal José M, Mangold Atilio J, Guglielmone Alberto A, Nava Santiago
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300, Rafaela, Santa Fe, Argentina.
Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte - Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay.
Exp Appl Acarol. 2018 Jan;74(1):107-116. doi: 10.1007/s10493-018-0208-4. Epub 2018 Jan 27.
This study was performed to determine the tick species that infest cattle and humans throughout an altitudinal gradient in the Yungas Biogeographic Province of Argentina. The presence of tick-borne bacteria of the genera Rickettsia, Ehrlichia and Borrelia in the collected ticks was also evaluated. Samples of ticks parasitizing cattle and humans were carried out in different seasons. Questing ticks (adults and nymphs) were collected from vegetation and analyzed to detect the presence of Rickettsia, Ehrlichia and Borrelia by a battery of different PCRs. Five species of hard ticks were found parasitizing cattle: Amblyomma sculptum, Amblyomma tonelliae, Amblyomma hadanii, Haemaphysalis juxtakochi and Ixodes pararicinus. Amblyomma sculptum (immature and adults), A. tonelliae (immature and adults), A. hadanii (larvae) and one nymph of I. pararicinus were found attached to humans. Rickettsia amblyommatis was detected in one nymph of A. hadanii. DNA of a Borrelia genospecies belonging to the B. burgdorferi s.l. complex (phylogenetically related to haplotypes previously reported in Ixodes aragaoi from Uruguay and I. pararicinus from Argentina) was detected in adults of I. pararicinus. Amblyomma sculptum and I. pararicinus appear to be the tick species more frequent on cattle in the YBP from Argentina, and A. sculptum and A. tonelliae, were the main ticks found attached to humans. The medical importance of the bacteria of the genus Rickettsia and Borrelia detected in this work remains unknown.
本研究旨在确定在阿根廷永加斯生物地理省沿海拔梯度侵袭牛和人类的蜱种。还评估了所采集蜱中属于立克次体属、埃立克体属和疏螺旋体属的蜱传细菌的存在情况。在不同季节采集寄生在牛和人类身上的蜱样本。从植被中收集寻觅蜱（成虫和若虫），并通过一系列不同的聚合酶链反应（PCR）分析以检测立克次体、埃立克体和疏螺旋体的存在。发现有五种硬蜱寄生在牛身上：刻点钝缘蜱、托氏钝缘蜱、哈氏钝缘蜱、近科氏血蜱和副里氏硬蜱。发现刻点钝缘蜱（未成熟个体和成虫）、托氏钝缘蜱（未成熟个体和成虫）、哈氏钝缘蜱（幼虫）以及一只副里氏硬蜱若虫附着在人类身上。在一只哈氏钝缘蜱若虫中检测到了安氏立克次体。在副里氏硬蜱成虫中检测到了一种属于伯氏疏螺旋体复合群的疏螺旋体属基因种的DNA（在系统发育上与先前在乌拉圭的阿拉戈硬蜱和阿根廷的副里氏硬蜱中报道的单倍型相关）。刻点钝缘蜱和副里氏硬蜱似乎是阿根廷永加斯生物地理省牛身上较常见的蜱种，而刻点钝缘蜱和托氏钝缘蜱是发现附着在人类身上的主要蜱种。在本研究中检测到的立克次体属和疏螺旋体属细菌的医学重要性尚不清楚。
