College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Jinju, South Korea.
Department of Animal Science, College of Agriculture, Gyeongsang National University, Jinju, South Korea.
Infect Immun. 2014 Sep;82(9):3845-54. doi: 10.1128/IAI.02141-14. Epub 2014 Jun 30.
Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description of the sequence analysis, bioactivity, and comparative expression analysis of chicken IL-17RA (chIL-17RA) in chickens infected with Salmonella and Eimeria, two major infectious agents of gastrointestinal diseases of poultry of economic importance. A full-length chIL-17RA cDNA with a 2,568-bp coding region was identified from chicken thymus cDNA. chIL-17RA shares ca. 46% identity with mammalian homologues and 29.2 to 31.5% identity with its piscine counterparts. chIL-17RA transcript expression was relatively high in the thymus and in the chicken macrophage cell line HD11. The chIL-17RA-specific small interfering RNA inhibits interleukin-6 (IL-6), IL-8, and IL-1β mRNA expression in chicken embryo fibroblast cells (but not in DF-1 cells) stimulated with chIL-17A or chIL-17F. Interaction between chIL-17RA and chIL-17A was confirmed by coimmunoprecipitation. Downregulation of chIL-17RA occurred in concanavalin A- or lipopolysaccharide-activated splenic lymphocytes but not in poly(I·C)-activated splenic lymphocytes. In Salmonella- and Eimeria-infected chickens, the expression levels of the chIL-17RA transcript were downregulated in intestinal tissues from chickens infected with two Eimeria species, E. tenella or E. maxima, that preferentially infect the cecum and jejunum, respectively. However, chIL-17RA expression was generally unchanged in Salmonella infection. These results suggest that chIL-17RA has an important role in mucosal immunity to intestinal intracellular parasite infections such as Eimeria infection.
白细胞介素-17A (IL-17A) 和 IL-17F 都是促炎细胞因子，通过受体信号在肠道稳态中发挥重要作用。这些细胞因子在鸡中已经得到了描述，但对它们的受体及其功能活性知之甚少。我们在这里首次描述了鸡白细胞介素-17 受体 A (chIL-17RA) 的序列分析、生物活性和比较表达分析，该受体在感染沙门氏菌和艾美耳球虫的鸡中发挥作用，这两种病原体是具有经济重要性的家禽胃肠道疾病的主要传染性病原体。从鸡胸腺 cDNA 中鉴定出一个全长的 chIL-17RA cDNA，其编码区为 2568bp。chIL-17RA 与哺乳动物同源物的相似度约为 46%，与鱼类同源物的相似度为 29.2%至 31.5%。chIL-17RA 的转录表达在胸腺和鸡巨噬细胞系 HD11 中相对较高。chIL-17RA 特异性的小干扰 RNA 抑制白细胞介素-6 (IL-6)、IL-8 和 IL-1β mRNA 在鸡胚成纤维细胞中的表达（但在 DF-1 细胞中不表达），这些细胞被 chIL-17A 或 chIL-17F 刺激。通过共免疫沉淀证实了 chIL-17RA 与 chIL-17A 的相互作用。刀豆球蛋白 A 或脂多糖激活的脾淋巴细胞中 chIL-17RA 的下调，但在聚肌苷酸-聚胞苷酸激活的脾淋巴细胞中没有下调。在感染沙门氏菌和艾美耳球虫的鸡中，两种艾美耳球虫，柔嫩艾美耳球虫或巨型艾美耳球虫，分别优先感染盲肠和空肠，感染这两种艾美耳球虫的鸡肠组织中 chIL-17RA 转录本的表达水平下调。然而，在沙门氏菌感染中，chIL-17RA 的表达通常不变。这些结果表明，chIL-17RA 在对肠道细胞内寄生虫感染（如艾美耳球虫感染）的黏膜免疫中具有重要作用。
