Hong Yeong Ho, Lillehoj Hyun S, Park Dong Woon, Lee Sung Hyen, Han Jae Yong, Shin Ji Hye, Park Myeung Sun, Kim Jin-Kyoo
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Building 1040, BARC-East, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Vet Immunol Immunopathol. 2008 Nov 15;126(1-2):1-8. doi: 10.1016/j.vetimm.2008.06.002. Epub 2008 Jun 17.
The chicken interleukin-17D was cloned from a testis cDNA library prepared from the Korean native chicken. The full-length chicken IL-17D (chIL-17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with a predicted molecular mass of 13.3kDa. Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76% identity, respectively, including six conserved cysteine residues present in the mammalian polypeptides. The chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung. Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus. Infected chickens also expressed greater levels of chIL-17D mRNA in CD4(+), CD8(+) and TCR1(+) intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2(+) cells. Treatment of CHCC-OU2 fibroblasts with chIL-17D recombinant protein induced the expression of IL-6 and IL-8. Collectively, these results suggest that chL-17D has structural and functional similarities to mammalian IL-17Ds and that it plays an important role in local gut innate immune responses during experimental coccidiosis.
鸡白细胞介素-17D是从韩国本地鸡的睾丸cDNA文库中克隆出来的。鸡白细胞介素-17D（chIL-17D）全长cDNA由348个核苷酸序列组成，编码一个116个氨基酸的开放阅读框，预测分子量为13.3kDa。将chIL-17D推导的氨基酸序列与人、小鼠和负鼠的同源蛋白进行比较，分别显示出64%、53%和76%的同一性，包括哺乳动物多肽中存在的六个保守半胱氨酸残基。chIL-17D基因转录本在多种组织中表达，在胰腺、胸腺和肺中表达水平最高。感染巨型艾美耳球虫后，chIL-17D mRNA水平在空肠、法氏囊、肺和脾脏中上调，但在胸腺中下降。感染的鸡在CD4(+)CD8(+)和TCR1(+)肠上皮内淋巴细胞中也表达更高水平的chIL-17D mRNA，而在TCR2(+)细胞中表达下降。用chIL-17D重组蛋白处理CHCC-OU2成纤维细胞可诱导IL-6和IL-8的表达。总体而言，这些结果表明chL-17D与哺乳动物IL-17D具有结构和功能上的相似性，并且在实验性球虫病期间在局部肠道固有免疫反应中起重要作用。
