Yunos Nina Yusrina Muhamad, Tan Wen-Si, Koh Chong-Lek, Sam Choon-Kook, Mohamad Nur Izzati, Tan Pui-Wan, Adrian Tan-Guan-Sheng, Yin Wai-Fong, Chan Kok-Gan
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore.
Sensors (Basel). 2014 Jun 30;14(7):11595-604. doi: 10.3390/s140711595.
Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-L-homoserine lactone (C8-HSL) and N-decanoyl-L-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.
群体感应（QS）是一种细菌细胞间通讯系统，它控制着与群体密度同步的群体感应介导基因。特定基因活性的调节取决于所产生的信号分子，即N-酰基高丝氨酸内酯（AHLs）。我们在此报告从马来西亚淡水样本中分离出的细菌菌株ND07所产生的AHLs的鉴定和特征。分子鉴定表明，菌株ND07与嗜麦芽窄食单胞菌密切聚类。嗜麦芽窄食单胞菌菌株ND07的培养上清液提取物激活了AHL生物传感器紫色色杆菌CV026。使用高分辨率三重四极杆液相色谱-质谱联用技术，证实嗜麦芽窄食单胞菌菌株ND07产生了N-辛酰-L-高丝氨酸内酯（C8-HSL）和N-癸酰-L-高丝氨酸内酯（C10-HSL）。据我们所知，这是关于嗜麦芽窄食单胞菌菌株ND07产生C10-HSL的首次报道。
