Tan Wen-Si, Yunos Nina Yusrina Muhamad, Tan Pui-Wan, Mohamad Nur Izzati, Adrian Tan-Guan-Sheng, Yin Wai-Fong, Chan Kok-Gan
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
Sensors (Basel). 2014 Jul 8;14(7):12104-13. doi: 10.3390/s140712104.
N-acylhomoserine lactones (AHL) plays roles as signal molecules in quorum sensing (QS) in most Gram-negative bacteria. QS regulates various physiological activities in relation with population density and concentration of signal molecules. With the aim of isolating marine water-borne bacteria that possess QS properties, we report here the preliminary screening of marine bacteria for AHL production using Chromobacterium violaceum CV026 as the AHL biosensor. Strain T33 was isolated based on preliminary AHL screening and further identified by using 16S rDNA sequence analysis as a member of the genus Vibrio closely related to Vibrio brasiliensis. The isolated Vibrio sp. strain T33 was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxodecanoyl)-L-homoserine lactone (3-oxo-C10 HSL) through high resolution tandem mass spectrometry analysis. We demonstrated that this isolate formed biofilms which could be inhibited by catechin. To the best of our knowledge, this is the first report that documents the production of these AHLs by Vibrio brasiliensis strain T33.
N-酰基高丝氨酸内酯（AHL）在大多数革兰氏阴性细菌的群体感应（QS）中作为信号分子发挥作用。群体感应调节与种群密度和信号分子浓度相关的各种生理活动。为了分离具有群体感应特性的海洋水生细菌，我们在此报告了以紫色色杆菌CV026作为AHL生物传感器对海洋细菌进行AHL产生的初步筛选。基于初步的AHL筛选分离出菌株T33，并通过16S rDNA序列分析进一步鉴定为与巴西弧菌密切相关的弧菌属成员。通过高分辨率串联质谱分析证实，分离出的弧菌属菌株T33产生N-己酰-L-高丝氨酸内酯（C6-HSL）和N-（3-氧代癸酰基）-L-高丝氨酸内酯（3-氧代-C10 HSL）。我们证明该分离株形成的生物膜可被儿茶素抑制。据我们所知，这是第一份记录巴西弧菌菌株T33产生这些AHL的报告。
